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MB Sample ID: SA330276
| Local Sample ID: | EV_AII |
| Subject ID: | SU003155 |
| Subject Type: | Plant |
| Subject Species: | Echium vulgare |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003155 |
| Subject Type: | Plant |
| Subject Species: | Echium vulgare |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| EV_AII | SA330276 | FL039076 | A | Location |
| EV_AII | SA330276 | FL039076 | rosette | Developmental Stage |
| EV_AII | SA330276 | FL039076 | neutral | Soil pH |
| EV_AII | SA330276 | FL039076 | G1 | Genetic cluster |
Collection:
| Collection ID: | CO003148 |
| Collection Summary: | Echium vulgare plants were collected in six locations in Austria. Four of the locations contained both rosette-stage and flowering plants, while two of the remaining locations contained only rosette-stage plants. Roots of the plants were collected and subsequently analyzed. |
| Sample Type: | Plant roots |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003164 |
| Treatment Summary: | After the collection, no additional treatment except for the cutting of roots was performed. The roots were split into parts for various microbiological analyses and parts for metabolomics analysis. |
Sample Preparation:
| Sampleprep ID: | SP003161 |
| Sampleprep Summary: | Plant roots were ground to a fine powder using a ball mill (Fritsch Pulverisette 0, Germany). Each powdered sample was weighed (70 mg) into microcentrifuge tubes, followed by extraction with 3 mL of methanol by ultrasound at 10% power for 3 h (Bandelin Sonorex Digital 10P, Berlin, Germany) and centrifugation for 10 minutes at 12.500 rpm (Hermle Z 216 MK, Wehingen, Germany). The supernatants were collected and subjected to UHPLC-HRMS analysis after filtering with 0.22 μm syringe filters. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | Room temperature |
Combined analysis:
| Analysis ID | AN004988 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Accela AS |
| Column | Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo LTQ Discovery Orbitrap |
| Ion Mode | POSITIVE |
| Units | Intensity |
Chromatography:
| Chromatography ID: | CH003768 |
| Instrument Name: | Thermo Accela AS |
| Column Name: | Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0 min 95A/5B, 1 min 50A/50B, 8 min 0A/100B, 13 min 0A/100B, 13.01 min 95A/5B, 16 min 95A/5B |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | Ultrapure water (0.1% formic acid) |
| Solvent B: | LCMS-grade methanol (0.1% formic acid) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004728 |
| Analysis ID: | AN004988 |
| Instrument Name: | Thermo LTQ Discovery Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The MS/MS data were obtained for the six most intense m/z peaks in each full scan, with the normalized collision energy set to 35 eV. The acquisition and initial processing of the data were by means of XcaliburTM (Thermo Scientific, USA) software, while data alignment and feature extraction were performed utilizing the XCMS Online platform (The Scripps Research Institute, USA). |
| Ion Mode: | POSITIVE |
