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MB Sample ID: SA330435
Local Sample ID: | Serum3MWN2negative |
Subject ID: | SU003158 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003158 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Serum3MWN2negative | SA330435 | FL039094 | Wild_Type | Genotype |
Serum3MWN2negative | SA330435 | FL039094 | tamoxifen, 3month | Treatment |
Collection:
Collection ID: | CO003151 |
Collection Summary: | serum and kidney were obtained from GCERRARaD females 3 days or 3 months post-tam (n=5/group) and age-matched wild-type females (n=4) |
Sample Type: | Kidney; Serum |
Treatment:
Treatment ID: | TR003167 |
Treatment Summary: | Mutant and wild-type female mice were treated with tamoxifen for 3 days or 3 months. |
Sample Preparation:
Sampleprep ID: | SP003164 |
Sampleprep Summary: | To extract metabolites, frozen kidney cortices were weighed, and ca 20 mg of each cortex was added to a tube containing 650 μL of 80% methanol (diluted in dH2O). Sample tubes were placed in a bead homogenizer for 1 minute at a frequency of 30/s. Samples were then placed in a -20℃ freezer for 10 minutes, centrifuged for 10 minutes at 15,000 RPM in 4℃, and the supernatant was moved to a new tube. The remaining pellets were then re-extracted with 400 μL of 80% methanol, following the above procedure of homogenization and centrifugation. The supernatant was then added to the supernatant pool obtained from the first extraction. The pellet was re-extracted for a third time using 350 μL of 80% MeOH and supernatant was again added to the pool from the first two extractions. The supernatant pool was placed at -20℃ for 15 minutes, centrifuged for 20 minutes at 15,000 RPM, and transferred to a new tube. The methanolic extract samples were speed-vacuumed and reconstituted in 70% acetonitrile (diluted in dH2O) containing 0.025% acetic acid at a protein concentration of 3μg/μL. For serum preparation, samples were diluted 10-fold with 70% acetonitrile (diluted in dH2O) containing 0.025% acetic acid, and centrifuged for 20 minutes at 20,000 g. |
Combined analysis:
Analysis ID | AN004992 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Agilent 1290 Infinity II |
Column | MicroSolv Cogent Diamond Hydride (150 × 2.1 mm, 4.0 um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6550 QTOF |
Ion Mode | UNSPECIFIED |
Units | abundance |
Chromatography:
Chromatography ID: | CH003771 |
Instrument Name: | Agilent 1290 Infinity II |
Column Name: | MicroSolv Cogent Diamond Hydride (150 × 2.1 mm, 4.0 um) |
Column Temperature: | 25 |
Flow Gradient: | 0-1 min, 99% B; 1-15 min, a linear gradient to 20% B; 15.1-29 min, 0% B; 29.1-37 min, 99% B |
Flow Rate: | 0.4mL/min |
Solvent A: | 6 uM edta and 0.025% acetic acid in isopropanol:H2O (50:50) |
Solvent B: | 6 uM edta and 5mM ammonium acetate in CH3CN: H2O (90:10) |
Chromatography Type: | HILIC |
MS:
MS ID: | MS004732 |
Analysis ID: | AN004992 |
Instrument Name: | Agilent 6550 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | The serum samples have negative and positive mode data. The kidney samples have negative mode data. MassHunter B10 MassHunter B10 MassHunter Profinder (B10) |
Ion Mode: | UNSPECIFIED |