Return to study ST003048 main page
MB Sample ID: SA331054
Local Sample ID: | SMFs-52 |
Subject ID: | SU003163 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003163 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
SMFs-52 | SA331054 | FL039175 | Non-EC | Group |
Collection:
Collection ID: | CO003156 |
Collection Summary: | Blood was collected through venipuncture and then centrifuged at 2000 g for 10 minutes. The serum was transferred to a microtube immediately and stored at -80°C. The samples of the EC and Non-EC groups were collected during a similar period from Dec. 2018 to Sep. 2021, and the serum metabolic fingerprints (SMFs) database was recorded in Jul. 2022 using the serum samples that underwent one freeze-thaw cycle. The pathologists were blinded to any information about SMFs analysis. |
Sample Type: | Blood (serum) |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR003172 |
Treatment Summary: | NA |
Sample Preparation:
Sampleprep ID: | SP003169 |
Sampleprep Summary: | 1.5 μL of serum samples (10-fold dilution) were spotted on a 384 polished steel plate and dried. |
Combined analysis:
Analysis ID | AN004999 |
---|---|
Analysis type | MS |
Chromatography type | None (Direct infusion) |
Chromatography system | NA |
Column | NA |
MS Type | MALDI |
MS instrument type | TOF |
MS instrument name | Bruker Autoflex speed TOF/TOF |
Ion Mode | POSITIVE |
Units | Peak intensity |
Chromatography:
Chromatography ID: | CH003777 |
Instrument Name: | NA |
Column Name: | NA |
Column Temperature: | NA |
Flow Gradient: | NA |
Flow Rate: | NA |
Solvent A: | NA |
Solvent B: | NA |
Chromatography Type: | None (Direct infusion) |
MS:
MS ID: | MS004739 |
Analysis ID: | AN004999 |
Instrument Name: | Bruker Autoflex speed TOF/TOF |
Instrument Type: | TOF |
MS Type: | MALDI |
MS Comments: | 10 mg of organic matrices were dissolved in 1 mL of TA30 solution (acetonitrile:0.1% TFA solution, 3:7, v/v), and the particle powder was dispersed in DIW with 1.0 mg/mL. 1.5 μL of either standard metabolite solutions or serum samples (10-fold dilution) were spotted on a 384 polished steel plate and dried. The serum samples were prepared randomly to minimize subjective bias for the SMFs database construction. Subsequently, matrix solution was added and dried before detection. Mass-to-charge ratio (m/z) calibration was performed using alanine, proline, glutamic acid, glucose, lactose, maltotriose, and thyroxine. The pulse frequency and laser shots per analysis were set to 1000 Hz and 2000, respectively. |
Ion Mode: | POSITIVE |