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MB Sample ID: SA332320
| Local Sample ID: | T3_6 |
| Subject ID: | SU003189 |
| Subject Type: | Synthetic sample |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003189 |
| Subject Type: | Synthetic sample |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| T3_6 | SA332320 | FL039344 | 2nM 3,5,3’-L-triiodothyronine | Standard |
Collection:
| Collection ID: | CO003182 |
| Collection Summary: | The presented study is on chemical standards |
| Sample Type: | synthetic |
Treatment:
| Treatment ID: | TR003198 |
| Treatment Summary: | no treatments were performed |
Sample Preparation:
| Sampleprep ID: | SP003195 |
| Sampleprep Summary: | standards were solubilized in 50/50 methanol/water with 10uM ammonium hydroxide |
Combined analysis:
| Analysis ID | AN005032 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Merck Supelco Ascentis Express C18 (150 x 2.1mm,2.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | NA |
Chromatography:
| Chromatography ID: | CH003803 |
| Methods Filename: | C18_LC_Conditions.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Merck Supelco Ascentis Express C18 (150 x 2.1mm,2.7um) |
| Column Temperature: | 30 |
| Flow Gradient: | 0–5 min: gradient was held at 5% B; 2–12.1 min: linear gradient of 5% to 95% B; 12.1–17.0 min: 95% B; 17.1–21.0 min: gradient was returned to 5% B |
| Flow Rate: | 0.25 ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004771 |
| Analysis ID: | AN005032 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | MS data acquisition was performed using a Q Exactive Orbitrap benchtop orbitrap mass spectrometer equipped with an Ion Max source and a HESI II probe (Thermo Fisher Scientific, San Jose, CA, USA)in positive ionization mode and a PRM acquisition with the resolution set at 17,500, the AGC target at 2 × 10^5, and the maximum injection time (Max IT) at 40 msec. Collision energies were set at 20,40,60 and 80 NCE. Inclusion list was 651.7973 m/z for T3 and 777.6940 m/z for T4. HESI conditions were as follows: sheath gas flow rate: 40 units; Aug gas flow rate: 10 units; sweet gas flow rate: 0; spray voltage: 3.5 kV (pos), 2.8 kV (neg); capillary temperature: 380 °C; S-lens RF: 60; Aux gas heater temperature: 420 °C. MS spectra were consolidated using the mzVault software, automatically thresholded for noise and recalibrated for mass error. |
| Ion Mode: | POSITIVE |
