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MB Sample ID: SA332320
Local Sample ID: | T3_6 |
Subject ID: | SU003189 |
Subject Type: | Synthetic sample |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003189 |
Subject Type: | Synthetic sample |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
T3_6 | SA332320 | FL039344 | 2nM 3,5,3’-L-triiodothyronine | Standard |
Collection:
Collection ID: | CO003182 |
Collection Summary: | The presented study is on chemical standards |
Sample Type: | synthetic |
Treatment:
Treatment ID: | TR003198 |
Treatment Summary: | no treatments were performed |
Sample Preparation:
Sampleprep ID: | SP003195 |
Sampleprep Summary: | standards were solubilized in 50/50 methanol/water with 10uM ammonium hydroxide |
Combined analysis:
Analysis ID | AN005032 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Vanquish |
Column | Merck Supelco Ascentis Express C18 (150 x 2.1mm,2.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | NA |
Chromatography:
Chromatography ID: | CH003803 |
Methods Filename: | C18_LC_Conditions.pdf |
Instrument Name: | Thermo Vanquish |
Column Name: | Merck Supelco Ascentis Express C18 (150 x 2.1mm,2.7um) |
Column Temperature: | 30 |
Flow Gradient: | 0–5 min: gradient was held at 5% B; 2–12.1 min: linear gradient of 5% to 95% B; 12.1–17.0 min: 95% B; 17.1–21.0 min: gradient was returned to 5% B |
Flow Rate: | 0.25 ml/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004771 |
Analysis ID: | AN005032 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | MS data acquisition was performed using a Q Exactive Orbitrap benchtop orbitrap mass spectrometer equipped with an Ion Max source and a HESI II probe (Thermo Fisher Scientific, San Jose, CA, USA)in positive ionization mode and a PRM acquisition with the resolution set at 17,500, the AGC target at 2 × 10^5, and the maximum injection time (Max IT) at 40 msec. Collision energies were set at 20,40,60 and 80 NCE. Inclusion list was 651.7973 m/z for T3 and 777.6940 m/z for T4. HESI conditions were as follows: sheath gas flow rate: 40 units; Aug gas flow rate: 10 units; sweet gas flow rate: 0; spray voltage: 3.5 kV (pos), 2.8 kV (neg); capillary temperature: 380 °C; S-lens RF: 60; Aux gas heater temperature: 420 °C. MS spectra were consolidated using the mzVault software, automatically thresholded for noise and recalibrated for mass error. |
Ion Mode: | POSITIVE |