Return to study ST003078 main page
MB Sample ID: SA332629
| Local Sample ID: | EM_N7_4_220720 |
| Subject ID: | SU003193 |
| Subject Type: | Bacteria |
| Subject Species: | Staphylococcus epidermidis |
| Taxonomy ID: | 1282 |
| Genotype Strain: | 19N/ICE25 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003193 |
| Subject Type: | Bacteria |
| Subject Species: | Staphylococcus epidermidis |
| Taxonomy ID: | 1282 |
| Genotype Strain: | 19N/ICE25 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| EM_N7_4_220720 | SA332629 | FL039389 | 19N | Sample source |
| EM_N7_4_220720 | SA332629 | FL039389 | 19N | Strain |
| EM_N7_4_220720 | SA332629 | FL039389 | 7.4 | initial pH |
| EM_N7_4_220720 | SA332629 | FL039389 | 2 | Time (h) |
| EM_N7_4_220720 | SA332629 | FL039389 | 1 | Replica |
Collection:
| Collection ID: | CO003186 |
| Collection Summary: | Hourly, an 800 µL aliquot was collected to measure OD600nm and the medium was separated from the cells by centrifugation at 5000 g, 5 min and 4°C. The supernatants were kept at -20°C until NMR analysis (for a maximum duration of 3 months). |
| Sample Type: | Bacterial cells |
| Storage Conditions: | -20℃ |
Treatment:
| Treatment ID: | TR003202 |
| Treatment Summary: | SE growth assays were performed for 19N and ICE25 strains at pH mimicking SE skin colonisation and blood infection (pH 5.5 and 7.4, respectively) in three independent replicates. A total of eight time-points were analysed: t0h collected immediately after inoculation, t2h-6h hourly during the exponential phase, t8h and t10h during the late exponential and beginning of the stationary phase. |
Sample Preparation:
| Sampleprep ID: | SP003199 |
| Sampleprep Summary: | After thawing, 540 µL of the media samples were transferred to 5 mm NMR tubes and 60 µL of the NMR buffer (1 M phosphate potassium buffer, 2 mM sodium azide, 3.22 mM 3-(Trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt (TSP), pH 7.0) was added. |
Analysis:
| MB Sample ID: | SA332629 |
| Analysis ID: | AN005036 |
| Analysis Type: | NMR |
| Num Factors: | 97 |
| Num Metabolites: | 47 |
| Units: | mM |
NMR:
| NMR ID: | NM000275 |
| Analysis ID: | AN005036 |
| Instrument Name: | Bruker Avance II+ 800 MHz |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 800 |
| NMR Probe: | 5 mm TXI-Z H/C/N/-D |
| NMR Solvent: | 90% H20 + 10% D2O |
| NMR Tube Size: | 5 mm |
| Shimming Method: | Topshim |
| Pulse Sequence: | noesygppr1d |
| Receiver Gain: | 57 |
| Chemical Shift Ref Cpd: | TSP |
| Temperature: | 25 |
| Number Of Scans: | 64 |
| Dummy Scans: | 4 |
