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MB Sample ID: SA332690
Local Sample ID: | EM_I7_7_221012 |
Subject ID: | SU003193 |
Subject Type: | Bacteria |
Subject Species: | Staphylococcus epidermidis |
Taxonomy ID: | 1282 |
Genotype Strain: | 19N/ICE25 |
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Subject:
Subject ID: | SU003193 |
Subject Type: | Bacteria |
Subject Species: | Staphylococcus epidermidis |
Taxonomy ID: | 1282 |
Genotype Strain: | 19N/ICE25 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
EM_I7_7_221012 | SA332690 | FL039450 | ICE25 | Sample source |
EM_I7_7_221012 | SA332690 | FL039450 | ICE25 | Strain |
EM_I7_7_221012 | SA332690 | FL039450 | 7.4 | initial pH |
EM_I7_7_221012 | SA332690 | FL039450 | 7 | Time (h) |
EM_I7_7_221012 | SA332690 | FL039450 | 1 | Replica |
Collection:
Collection ID: | CO003186 |
Collection Summary: | Hourly, an 800 µL aliquot was collected to measure OD600nm and the medium was separated from the cells by centrifugation at 5000 g, 5 min and 4°C. The supernatants were kept at -20°C until NMR analysis (for a maximum duration of 3 months). |
Sample Type: | Bacterial cells |
Storage Conditions: | -20℃ |
Treatment:
Treatment ID: | TR003202 |
Treatment Summary: | SE growth assays were performed for 19N and ICE25 strains at pH mimicking SE skin colonisation and blood infection (pH 5.5 and 7.4, respectively) in three independent replicates. A total of eight time-points were analysed: t0h collected immediately after inoculation, t2h-6h hourly during the exponential phase, t8h and t10h during the late exponential and beginning of the stationary phase. |
Sample Preparation:
Sampleprep ID: | SP003199 |
Sampleprep Summary: | After thawing, 540 µL of the media samples were transferred to 5 mm NMR tubes and 60 µL of the NMR buffer (1 M phosphate potassium buffer, 2 mM sodium azide, 3.22 mM 3-(Trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt (TSP), pH 7.0) was added. |
Analysis:
MB Sample ID: | SA332690 |
Analysis ID: | AN005036 |
Analysis Type: | NMR |
Num Factors: | 97 |
Num Metabolites: | 47 |
Units: | mM |
NMR:
NMR ID: | NM000275 |
Analysis ID: | AN005036 |
Instrument Name: | Bruker Avance II+ 800 MHz |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Spectrometer Frequency: | 800 |
NMR Probe: | 5 mm TXI-Z H/C/N/-D |
NMR Solvent: | 90% H20 + 10% D2O |
NMR Tube Size: | 5 mm |
Shimming Method: | Topshim |
Pulse Sequence: | noesygppr1d |
Receiver Gain: | 57 |
Chemical Shift Ref Cpd: | TSP |
Temperature: | 25 |
Number Of Scans: | 64 |
Dummy Scans: | 4 |