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MB Sample ID: SA332987
Local Sample ID: | Norm. Area 088_0009_01B_R_TM50xWTxCMLxTAx72x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F322) |
Subject ID: | SU003206 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003206 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Norm. Area 088_0009_01B_R_TM50xWTxCMLxTAx72x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F322) | SA332987 | FL039526 | T cells | Sample source |
Norm. Area 088_0009_01B_R_TM50xWTxCMLxTAx72x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F322) | SA332987 | FL039526 | KO | Genotype |
Collection:
Collection ID: | CO003199 |
Collection Summary: | Tcells from miR142 WT and KO BCR ABL mice were isolated and subjected to resting or 24h activation. |
Sample Type: | T-cells |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR003215 |
Treatment Summary: | CD3+ T cells were sorted by flow cytometry from the spleen of Mir142−/−BCR-ABL (KO-T, n=9) and Mir142+/+BCR-ABL (WT-T, n=9) mice (BCR-ABL expression was induced for 2 weeks by tetracycline withdrawal). T cells from 5 mice per group (6x106/sample) were snap-frozen in liquid nitrogen as resting T cells. T cells from 4 mice per group were cultured in the presence of activating anti-CD3/CD28 antibody-coated beads for 24 hours before snap freezing (3x106/sample, activated). Metabolite extraction was performed on resting (6x106/sample, n=5 sample per group) and activated (3x106/sample, n=4 sample per group) T cells from both Mir142−/−BCR-ABL and Mir142+/+BCR-ABL mice and subjected to untargeted metabolomics as described previously. |
Sample Preparation:
Sampleprep ID: | SP003212 |
Sampleprep Summary: | Metabolite extraction on T cells were performed using 70% acetonitrile/methanol and 30% water. The metabolite extracts were subjected to untargeted metabolomics using HILIC and reversed phase chromatographies in positive and negative modes. |
Processing Storage Conditions: | 4℃ |
Extraction Method: | Polar solvent extraction |
Extract Storage: | -80℃ |
Sample Derivatization: | none |
Combined analysis:
Analysis ID | AN005056 | AN005057 | AN005058 | AN005059 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | Thermo Hypersil Gold C18 (150x 2.1mm, 3uM) | Thermo Hypersil Gold C18 (150x 2.1mm, 3uM) | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | Peak area | Peak area | Peak area | Peak area |
Chromatography:
Chromatography ID: | CH003819 |
Chromatography Summary: | Reversed phase chromatography |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Thermo Hypersil Gold C18 (150x 2.1mm, 3uM) |
Column Temperature: | 45 |
Flow Gradient: | 0%A for 1 min, 50% B for 4 min, 98%B for 3 min, 0% A for 5min |
Flow Rate: | 0.3ml/min |
Injection Temperature: | 4 |
Solvent A: | Water, 0.1% formic acid |
Solvent B: | Acetonitrile, 0.1% formic acid |
Transferline Temperature: | 275 |
Target Sample Temperature: | 4 |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003820 |
Chromatography Summary: | HILIC chromatography |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
Column Temperature: | 45 |
Flow Gradient: | 99%B for 1min, 85%B for 2min, 75%B for 3 min, 30%B for 3 min, 99%B for 5 min |
Flow Rate: | 0.35ml/min |
Injection Temperature: | 4 |
Solvent A: | 10mM ammonium acetate, 95% Water, 5% Acetonitrile, 0.1% acetic acid |
Solvent B: | 10mM ammonium acetate, 95% Acetonitrile, 95% Water, 0.1% acetic acid |
Transferline Temperature: | 275 |
Target Sample Temperature: | 4 |
Chromatography Type: | HILIC |
MS:
MS ID: | MS004794 |
Analysis ID: | AN005056 |
Instrument Name: | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Xcalibur software for Data dependent acquisition Compound discoverer 3.2 for data processing Skyline for data evaluation |
Ion Mode: | POSITIVE |
MS ID: | MS004795 |
Analysis ID: | AN005057 |
Instrument Name: | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Xcalibur software for Data dependent acquisition Compound discoverer 3.2 for data processing Skyline for data evaluation |
Ion Mode: | NEGATIVE |
MS ID: | MS004796 |
Analysis ID: | AN005058 |
Instrument Name: | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Xcalibur software for Data dependent acquisition Compound discoverer 3.2 for data processing Skyline for data evaluation |
Ion Mode: | POSITIVE |
MS ID: | MS004797 |
Analysis ID: | AN005059 |
Instrument Name: | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Xcalibur software for Data dependent acquisition Compound discoverer 3.2 for data processing Skyline for data evaluation |
Ion Mode: | NEGATIVE |