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MB Sample ID: SA333628
Local Sample ID: | 52 |
Subject ID: | SU003220 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003220 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
52 | SA333628 | FL039614 | Plasma | Sample source |
52 | SA333628 | FL039614 | Transgenic | Genotype |
Collection:
Collection ID: | CO003213 |
Collection Summary: | Blood was collected in EDTA-coated tubes, and plasma was obtained through centrifugation (7000 x g, 7 min, 4°C), snap frozen in liquid nitrogen and stored at -80°C until further use. |
Sample Type: | Blood (plasma) |
Treatment:
Treatment ID: | TR003229 |
Treatment Summary: | Control mice (wild-type) and mice expressing hM3Dq in GFRAL neurons (transgenic) were given CNO (1 mg/kg) i.p. and bled 6 hours later for NMR metabolomics. |
Sample Preparation:
Sampleprep ID: | SP003226 |
Sampleprep Summary: | Plasma was thawed at room temperature for 15 min before transferring 150 µl to pre-washed ultrafiltration tubes (Amicon Ultra 0.5 ml, 3k MWCO, Merck Millipore) which were spun at 14000 x g, at 4°C for 90 min. 90 µl of each filtrate was mixed with 90 µl buffer (75 mM sodium phosphate pH 7.4, 0.1% w/v sodium azide, 3.66 mM 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid (TSPd4), in 20% v/v D2O) and transferred to 3 mm SampleJet NMR-tubes. |
Analysis:
MB Sample ID: | SA333628 |
Analysis ID: | AN005084 |
Analysis Type: | NMR |
Num Factors: | 2 |
Num Metabolites: | 49 |
Units: | mM |
NMR:
NMR ID: | NM000276 |
Analysis ID: | AN005084 |
Instrument Name: | Bruker Neo |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Spectrometer Frequency: | 599.75 MHz |