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MB Sample ID: SA338037
Local Sample ID: | 2DG_4 |
Subject ID: | SU003235 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003235 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
2DG_4 | SA338037 | FL040040 | Brain | Sample source |
2DG_4 | SA338037 | FL040040 | 2DG | Treatment |
Collection:
Collection ID: | CO003228 |
Collection Summary: | Animals were euthanized by CO2 inhalation 16 hours following the final injection and transcardially perfused with ice-cold PBS. Whole brains and spinal cords were then excised, flash frozen in liquid N2, and stored at –80°C for biochemical analysis. |
Sample Type: | Brain |
Treatment:
Treatment ID: | TR003244 |
Treatment Summary: | Mice were administered i.p. injections of saline (vehicle) or 4 g/kg 2DG (Cayman Chemical) twice weekly for six weeks. |
Sample Preparation:
Sampleprep ID: | SP003242 |
Sampleprep Summary: | Metabolites from equal amounts of frontal cortex tissue were rapidly extracted in 80% ice-cold methanol. Extracted samples were vortexed twice, cleared by centrifugation at 14,000 x g for 20 minutes at 4°C, and stored at -80°C. |
Combined analysis:
Analysis ID | AN005112 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | Merck SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | peak intensity |
Chromatography:
Chromatography ID: | CH003868 |
Instrument Name: | Thermo Vanquish |
Column Name: | Merck SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
Column Temperature: | 30 |
Flow Gradient: | 85% to 30% A in 20 min followed by a wash with 30% A and re-equilibration at 85% A |
Flow Rate: | 150 μL/min |
Solvent A: | 100% acetonitrile |
Solvent B: | 100% water; 0.1% ammonium hydroxide; 20 mM ammonium acetate |
Chromatography Type: | HILIC |
MS:
MS ID: | MS004849 |
Analysis ID: | AN005112 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | 1. A Sequant ZIC-pHILIC column (2.1 mm i.d. × 150 mm, particle size of 5 µm, Millipore Sigma) was used for separation of metabolites. A 2.1 × 20 mm guard column with the same packing material was used for protection of the analytical column. Flow rate was set at 150 μL/min. Buffers consisted of 100% acetonitrile for mobile phase A, and 100% acetonitrile for mobile phase B. The chromatographic gradient ran from 85% to 30% A in 20 min followed by a wash with 30% A and re-equilibration at 85% A. Column temperature was 30 C. |
Ion Mode: | UNSPECIFIED |