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MB Sample ID: SA338958
Local Sample ID: | HL_WTHFD_1 |
Subject ID: | SU003244 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003244 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
HL_WTHFD_1 | SA338958 | FL040138 | Heart | Sample source |
HL_WTHFD_1 | SA338958 | FL040138 | WT | Genotype |
HL_WTHFD_1 | SA338958 | FL040138 | High Fat Diet | Treatment |
HL_WTHFD_1 | SA338958 | FL040138 | F | Sex |
HL_WTHFD_1 | SA338958 | FL040138 | 75 | Age |
Collection:
Collection ID: | CO003237 |
Collection Summary: | Murine heart tissue was excised and snap frozen in liquid N2. |
Sample Type: | Heart |
Treatment:
Treatment ID: | TR003253 |
Treatment Summary: | CHCHD10 WT and CHCHD10 S55L heterozygous mice were treated with either a control diet (70% Carbohydrate, 20% Protein, 10% Fat) or High Fat Diet (60% Fat, 20% Protein, 20% Carbohydrate) in utero until 75 days of age. |
Sample Preparation:
Sampleprep ID: | SP003251 |
Sampleprep Summary: | In brief, 1 ml of 90% isopropanol was added to 15 mg of homogenized heart tissue. Samples were vortexed for 2 min, sonicated for 1 min using a probe sonicator and centrifuged at 15,000 g for 10 min. The supernatant was collected and dried down using a SpeedVac. The dried samples were reconstituted using acetonitrile/isopropanol/water 65:30:5 containing stable isotope labeled internal lipid standards (Splash Lipidomix, Avanti Polar Lipids) prior to LC-MS analysis. |
Combined analysis:
Analysis ID | AN005126 | AN005127 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish |
Column | Imtakt Cadenza CD-C18 (150 x 2.1mm, 3um) | Imtakt Cadenza CD-C18 (150 x 2.1mm, 3um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak Intensity | Peak Intensity |
Chromatography:
Chromatography ID: | CH003879 |
Methods Filename: | Method_Lipidomics.pdf |
Instrument Name: | Thermo Vanquish |
Column Name: | Imtakt Cadenza CD-C18 (150 x 2.1mm, 3um) |
Column Temperature: | 35 |
Flow Gradient: | 0–1.5 min, 32% B; 1.5-4 min, 32-45% B; 4-5 min, 45-52% B; 5-8 min, 52-58% B; 8-11 min, 58-66% B; 11-14 min, 66-70% B; 14-18 min, 70-75% B; 21-25 min, isocratic 97% B, 25-25.1 min 97-32% B |
Flow Rate: | 200 µl/min |
Solvent A: | 60% acetonitrile/40% water; 10 mM ammonium formate; 0.1% formic acid |
Solvent B: | 90% isopropanol/10% acetonitrile; 10 mM ammonium formate; 0.1% formic acid |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004862 |
Analysis ID: | AN005126 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | A data-dependent mass spectrometric acquisition method was used for lipid identification. In this method, each MS survey scan was followed by up to 10 MS/MS scans performed on the most abundant ions. Data was acquired in positive and negative mode in separate runs. The following electrospray parameters were used: spray voltage 3.0 kV, heated capillary temperature 350 °C, HESI probe temperature 350 °C, sheath gas, 35 units; auxiliary gas 10 units. For MS scans: resolution, 70,000 (at m/z 200); automatic gain control target, 3e6; maximum injection time, 200 ms; scan range, 250-1800 m/z. For MS/MS scans: resolution, 17,500 (at 200 m/z); automatic gain control target, 1e5 ions; maximum injection time, 75 ms; isolation window, 1 m/z; NCE, stepped 20,30 and 40. The LC-MS results were processed using MS-DIAL software (version 4.9) for lipid identification and relative quantitation. |
Ion Mode: | POSITIVE |
Analysis Protocol File: | Method_Lipidomics.pdf |
MS ID: | MS004863 |
Analysis ID: | AN005127 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | A data-dependent mass spectrometric acquisition method was used for lipid identification. In this method, each MS survey scan was followed by up to 10 MS/MS scans performed on the most abundant ions. Data was acquired in positive and negative mode in separate runs. The following electrospray parameters were used: spray voltage 3.0 kV, heated capillary temperature 350 °C, HESI probe temperature 350 °C, sheath gas, 35 units; auxiliary gas 10 units. For MS scans: resolution, 70,000 (at m/z 200); automatic gain control target, 3e6; maximum injection time, 200 ms; scan range, 250-1800 m/z. For MS/MS scans: resolution, 17,500 (at 200 m/z); automatic gain control target, 1e5 ions; maximum injection time, 75 ms; isolation window, 1 m/z; NCE, stepped 20,30 and 40. The LC-MS results were processed using MS-DIAL software (version 4.9) for lipid identification and relative quantitation. |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | Method_Lipidomics.pdf |