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MB Sample ID: SA341163
Local Sample ID: | CRC-tissue-P5.105.fid# |
Subject ID: | SU003272 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003272 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
CRC-tissue-P5.105.fid# | SA341163 | FL040366 | Colorectal tissue | Sample source |
CRC-tissue-P5.105.fid# | SA341163 | FL040366 | early para-CRC | Factor |
CRC-tissue-P5.105.fid# | SA341163 | FL040366 | CRC | Factor |
Collection:
Collection ID: | CO003265 |
Collection Summary: | Resected colorectal cancer tissues and their corresponding non-cancerous tissues were frozen in liquid nitrogen for 10s before being transferred to cryogenic vials and stored at −80°C until metabolite extraction. |
Sample Type: | colorectal tissue |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR003281 |
Treatment Summary: | None |
Sample Preparation:
Sampleprep ID: | SP003279 |
Sampleprep Summary: | Frozen tissue samples (approximately 300 mg) were thawed at 25°C, then sectioned and homogenized at 16,000 rpm for 80 seconds in a solution consisting of 0.6 mL distilled water and 1.2 mL methanol. Subsequently, chloroform (1.2 mL) and distilled water (1.2 mL) were added and the sample was vortexed for 60 seconds. Finally, samples were incubated on ice for 15 mins, followed by centrifugation at 2000 rpm for 5 mins. The resulting supernatant was washed with nitrogen and evaporated before incubating under vacuum for at least 18 hours. The subsequent lyophilized powder was dissolved in 550 μL PBS/D2O buffer (0.1 M, pH 7.4) and 50 μL TSP/D2O stock solution, and after centrifugation at 10,000 rpm for 5 mins,500 μL of the supernatant was transferred to an NMR tube (5 mm size) for 1H NMR spectroscopy analysis. |
Analysis:
MB Sample ID: | SA341163 |
Analysis ID: | AN005176 |
Analysis Type: | NMR |
Results File: | ST003155_AN005176_Results.txt |
Units: | peak area |
NMR:
NMR ID: | NM000279 |
Analysis ID: | AN005176 |
Instrument Name: | Bruker Avance III |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Spectrometer Frequency: | 600 MHz |