Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA341182

Local Sample ID:	CRC-tissue-P5.107.fid#
Subject ID:	SU003272
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Subject:

Subject ID:	SU003272
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
CRC-tissue-P5.107.fid#	SA341182	FL040369	Colorectal tissue	Sample source
CRC-tissue-P5.107.fid#	SA341182	FL040369	early para-CRC	Factor
CRC-tissue-P5.107.fid#	SA341182	FL040369	rectum	Factor

Collection:

Collection ID:	CO003265
Collection Summary:	Resected colorectal cancer tissues and their corresponding non-cancerous tissues were frozen in liquid nitrogen for 10s before being transferred to cryogenic vials and stored at −80°C until metabolite extraction.
Sample Type:	colorectal tissue
Storage Conditions:	-80℃

Treatment:

Treatment ID:	TR003281
Treatment Summary:	None

Sample Preparation:

Sampleprep ID:	SP003279
Sampleprep Summary:	Frozen tissue samples (approximately 300 mg) were thawed at 25°C, then sectioned and homogenized at 16,000 rpm for 80 seconds in a solution consisting of 0.6 mL distilled water and 1.2 mL methanol. Subsequently, chloroform (1.2 mL) and distilled water (1.2 mL) were added and the sample was vortexed for 60 seconds. Finally, samples were incubated on ice for 15 mins, followed by centrifugation at 2000 rpm for 5 mins. The resulting supernatant was washed with nitrogen and evaporated before incubating under vacuum for at least 18 hours. The subsequent lyophilized powder was dissolved in 550 μL PBS/D2O buffer (0.1 M, pH 7.4) and 50 μL TSP/D2O stock solution, and after centrifugation at 10,000 rpm for 5 mins,500 μL of the supernatant was transferred to an NMR tube (5 mm size) for 1H NMR spectroscopy analysis.

Sampleprep ID:

SP003279

Sampleprep Summary:

Frozen tissue samples (approximately 300 mg) were thawed at 25°C, then sectioned and homogenized at 16,000 rpm for 80 seconds in a solution consisting of 0.6 mL distilled water and 1.2 mL methanol. Subsequently, chloroform (1.2 mL) and distilled water (1.2 mL) were added and the sample was vortexed for 60 seconds. Finally, samples were incubated on ice for 15 mins, followed by centrifugation at 2000 rpm for 5 mins. The resulting supernatant was washed with nitrogen and evaporated before incubating under vacuum for at least 18 hours. The subsequent lyophilized powder was dissolved in 550 μL PBS/D2O buffer (0.1 M, pH 7.4) and 50 μL TSP/D2O stock solution, and after centrifugation at 10,000 rpm for 5 mins,500 μL of the supernatant was transferred to an NMR tube (5 mm size) for 1H NMR spectroscopy analysis.

Analysis:

MB Sample ID:	SA341182
Analysis ID:	AN005176
Analysis Type:	NMR
Results File:	ST003155_AN005176_Results.txt
Units:	peak area

NMR:

NMR ID:	NM000279
Analysis ID:	AN005176
Instrument Name:	Bruker Avance III
Instrument Type:	FT-NMR
NMR Experiment Type:	1D-1H
Spectrometer Frequency:	600 MHz