Return to study ST003175 main page
MB Sample ID: SA343209
| Local Sample ID: | Mul1(-/-)_TZ 39 |
| Subject ID: | SU003294 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Wild type and Mul1(+/+) mice |
| Age Or Age Range: | 8 weeks |
| Gender: | Male and female |
| Animal Light Cycle: | 12-hour/12-hour light/dark cycle |
| Animal Feed: | Research Diets D12492 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003294 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Wild type and Mul1(+/+) mice |
| Age Or Age Range: | 8 weeks |
| Gender: | Male and female |
| Animal Light Cycle: | 12-hour/12-hour light/dark cycle |
| Animal Feed: | Research Diets D12492 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Mul1(-/-)_TZ 39 | SA343209 | FL040569 | Mul1(-/-) | Genotype |
| Mul1(-/-)_TZ 39 | SA343209 | FL040569 | Liver | Sample source |
Collection:
| Collection ID: | CO003287 |
| Collection Summary: | Liver tissue was obtained from Mul1(+/+) and Mul1(-/-) mice following a HFD for 16 weeks and stained with H&E or Oil Red Oil. |
| Sample Type: | Liver |
| Storage Conditions: | -20℃ |
Treatment:
| Treatment ID: | TR003303 |
| Treatment Summary: | Liver tissue was obtained from Mul1(+/+) and Mul1(-/-) mice following a HFD for 16 weeks and stained with H&E or Oil Red Oil. |
Sample Preparation:
| Sampleprep ID: | SP003301 |
| Sampleprep Summary: | liver tissues from mice were fixed overnight in 10% neutral formalin and embedded in paraffin. Paraffin-embedded tissues were cut into sections and stained with hematoxylin and eosin (H&E) for assessment of liver histology. Liver tissue from Mul1(+/+) and Mul1(-/-) mice on HFD were subjected to the Folch extraction in order to separate lipids and polar metabolites. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -20℃ |
Combined analysis:
| Analysis ID | AN005211 | AN005212 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | A.U. | A.U. |
Chromatography:
| Chromatography ID: | CH003942 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0−0.5 min, 60% B; 0.5−2 min, 60−95% B; 2−17 min, 95% B; 17−17.5 min, 95−60% B; 17.5−20 min, 60% B |
| Flow Rate: | 350 µl/min |
| Solvent A: | 60:40 acetonitrile:water; 10mM ammonium formate; 0.1% formic acid |
| Solvent B: | 90:8:2 2-propanol:acetonitrile:water; 10mM ammonium formate; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004944 |
| Analysis ID: | AN005211 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The aqueous phase was analyzed using the Thermo Q-Exactive Orbitrap mass spectrometer with Dionex UHPLC and autosampler. All samples were analyzed in positive and negative simultaneously with heated electrospray ionization (HESI) with a mass resolution of 35,000 at m/z 200 as separate injections. The polar metabolites were analyzed on Thermo Q-Exactive Orbitrap mass spectrometer equipped with UHPLC. Data were analyzed with MZmine and features were aligned for identification across samples. The metabolites were searched against the Southeastern Center for Integrated Metabolomics (SECIM) metabolite library using retention time and corresponding mass spectral data. Lipids isolated from Folch extraction procedure were also analyzed by the same instrument. Lipidomics data were analyzed using the LipidMatch software and identified lipid entities were exported in a tabular form. |
| Ion Mode: | POSITIVE |
| MS ID: | MS004945 |
| Analysis ID: | AN005212 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The aqueous phase was analyzed using the Thermo Q-Exactive Orbitrap mass spectrometer with Dionex UHPLC and autosampler. All samples were analyzed in positive and negative simultaneously with heated electrospray ionization (HESI) with a mass resolution of 35,000 at m/z 200 as separate injections. The polar metabolites were analyzed on Thermo Q-Exactive Orbitrap mass spectrometer equipped with UHPLC. Data were analyzed with MZmine and features were aligned for identification across samples. The metabolites were searched against the Southeastern Center for Integrated Metabolomics (SECIM) metabolite library using retention time and corresponding mass spectral data. Lipids isolated from Folch extraction procedure were also analyzed by the same instrument. Lipidomics data were analyzed using the LipidMatch software and identified lipid entities were exported in a tabular form. |
| Ion Mode: | NEGATIVE |
