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MB Sample ID: SA346533
| Local Sample ID: | 20210111_MP_74 |
| Subject ID: | SU003300 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003300 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 20210111_MP_74 | SA346533 | FL040683 | Plasma | Sample source |
| 20210111_MP_74 | SA346533 | FL040683 | Postmenopause | Condition |
| 20210111_MP_74 | SA346533 | FL040683 | Depression | Symptom |
Collection:
| Collection ID: | CO003293 |
| Collection Summary: | Blood samples were colected after 12 hours overnight fast and centrifuged 20 min, 3000 RPM, 4ºC |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR003309 |
| Treatment Summary: | No treatment. Analyses of pre- and post-menopause women with or without depressive symptons |
Sample Preparation:
| Sampleprep ID: | SP003307 |
| Sampleprep Summary: | Aliquots of 50 µL of plasma were added into a 96-deep well plate cell to which 1 µL of thiol-derivatization solution (200 mM N-ethylmaleimide (NEM) + 2 mM citric acid) was added. Subsequently, 200 µL of precipitation solution (acetonitrile/isopropanol (7:3, v/v) + 1% formic acid + isotopically labelled internal standards) were added. After vortex agitation for 10 min. and refrigeration at -20ºC for 10 minutes, the samples were centrifuged (14.000 rpm for 10 minutes at 4°C). |
Combined analysis:
| Analysis ID | AN005225 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu Nexera X2 |
| Column | Merck Discovery HS F5 (PFP) (150 x 2.1mm,3um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Shimadzu LCMS-IT-TOF |
| Ion Mode | UNSPECIFIED |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003952 |
| Chromatography Summary: | Shimadzu Nexera X2 was used for chromatography, whereas Shimadzu LCMS-IT-TOF was used for MS runs |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Merck Discovery HS F5 (PFP) (150 x 2.1mm,3um) |
| Column Temperature: | 40ºC |
| Flow Gradient: | from 100% A to 5% A in 15 min., maintained 5% A for 5 min |
| Flow Rate: | 0.25 mL/min for 20 minutes |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004958 |
| Analysis ID: | AN005225 |
| Instrument Name: | Shimadzu LCMS-IT-TOF |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | polarity switching was used |
| Ion Mode: | UNSPECIFIED |
