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MB Sample ID: SA346830
| Local Sample ID: | CFT073_LB |
| Subject ID: | SU003306 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003306 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| CFT073_LB | SA346830 | FL040706 | LB_cells | Factor |
| CFT073_LB | SA346830 | FL040706 | media | Sample source |
Collection:
| Collection ID: | CO003299 |
| Collection Summary: | Media supernatant from overnight cultures. After overnight growth, cells were pelletized and media aspirated for extraction |
| Sample Type: | Media |
Treatment:
| Treatment ID: | TR003315 |
| Treatment Summary: | Media blanks were aliquoted and incubated overnight at 37C. Cells were innoculated into designated media and grown overnight for 16hrs at 37C before harvesting |
Sample Preparation:
| Sampleprep ID: | SP003313 |
| Sampleprep Summary: | 1 part media with 9 parts 75:25:0.2 ACN:MetOH:Formic Acid with internal val-d8 and phe-d8 standards; vortexed, centrifuged, supernatant removed and run on LC-MS |
Combined analysis:
| Analysis ID | AN005235 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1260 Infinity HPLC |
| Column | Waters Atlantis HILIC (150 x 2.1mm,3um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6530 QTOF |
| Ion Mode | POSITIVE |
| Units | ion counts |
Chromatography:
| Chromatography ID: | CH003962 |
| Instrument Name: | Agilent 1260 Infinity HPLC |
| Column Name: | Waters Atlantis HILIC (150 x 2.1mm,3um) |
| Column Temperature: | 30 |
| Flow Gradient: | 0.5 minute at 5% mobile phase B followed by a linear gradient to 40% mobile phase B over 10 minutes and then maintained isocratically for 4.5 minutes before returning to 5% mobile phase B by gradient over 2 minutes |
| Flow Rate: | 250 μL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004968 |
| Analysis ID: | AN005235 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Raw data from the LC–MS were analyzed using Agilent MassHunter Qualitative Analysis 10.0 software. |
| Ion Mode: | POSITIVE |
