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MB Sample ID: SA347858
| Local Sample ID: | 452 |
| Subject ID: | SU003308 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
| Human Race: | Asian |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003308 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
| Human Race: | Asian |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 452 | SA347858 | FL040844 | Septic shock | Subject_ID |
| 452 | SA347858 | FL040844 | Serum | Sample source |
Collection:
| Collection ID: | CO003301 |
| Collection Summary: | Adult patients who visited the emergency department of Taipei Veterans General Hospital between Nov. 2018 and Feb. 2019 were prospectively enrolled with approval from the Institutional Review Board. Blood samples were obtained during routine clinical testing upon admission before initial treatment. These samples were collected in serum separator tubes, left to clot at room temperature for 1 h, and then centrifuged at 3000 rpm at 4 °C for 20 min. The resulting supernatant sera were promptly stored at -80°C before subsequent analysis. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR003317 |
| Treatment Summary: | The samples detailed in this study did not undergo any treatment. |
Sample Preparation:
| Sampleprep ID: | SP003315 |
| Sampleprep Summary: | An aliquot of 5uL serum was extracted by 50 µL of methanol. After vortexing for 10 s, samples were centrifuged for 2 min at 12,000 rpm to precipitate proteins, and the supernatant was collected for mass spectrometry-based metabolomics analysis. |
Combined analysis:
| Analysis ID | AN005237 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH003964 |
| Chromatography Summary: | Chromatography methods for serum extract |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 30 °C |
| Flow Gradient: | 0-0.5 min, 95% B; 0.5-11 min, 95-50% B; 11-14 min, 50% B; 14-14.5 min, 50-95% B; 14.5-20 min, 95% B |
| Flow Rate: | 0.3 mL/min |
| Sample Injection: | 5 μL |
| Solvent A: | 100% water; 0.1% formic acid; 5 mM ammonium acetate |
| Solvent B: | 90% acetonitrile/10% water; 0.1% formic acid; 5 mM ammonium acetate |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004970 |
| Analysis ID: | AN005237 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Compound Discoverer 2.1 software (Thermo Fisher Scientific) was used for peak area integration. |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 250 °C |
| Ion Source Temperature: | 200 °C |
| Ion Spray Voltage: | 3.5 kV |
| Source Temperature: | 200 °C |
| Spray Voltage: | 3.5 kV |
