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MB Sample ID: SA353378
| Local Sample ID: | MI 3d-9 |
| Subject ID: | SU003363 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003363 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| MI 3d-9 | SA353378 | FL041186 | Spleen | Sample source |
| MI 3d-9 | SA353378 | FL041186 | MI 3d | treatment |
Collection:
| Collection ID: | CO003356 |
| Collection Summary: | Spleen samples from mice subjected to myocardial infarction (MI) surgery were extracted with liquid–liquid extraction. |
| Sample Type: | Spleen |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003372 |
| Treatment Summary: | No treatment |
Sample Preparation:
| Sampleprep ID: | SP003370 |
| Sampleprep Summary: | 50 mg of spleen tissues was homogenized with methanol (2% formic acid and 0.01 mol/L BHT) spiked with internal standard mixture. After centrifugation, the supernatant was added to water and ethylacetate, and the sample was mixed and centrifuged at 12,000 × g. The upper organic phase was transferred to a new tube, and the water phase was extracted again. The organic phase was combined and then evaporated to dryness and further dissolved in 100 μL of 30% acetonitrile. After vigorous mixing, samples were filtered by use of centrifuge tube filters (nylon membrane, 0.22 μm). |
Combined analysis:
| Analysis ID | AN005314 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTRAP |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | NEGATIVE |
| Units | ng/mg of tissue |
Chromatography:
| Chromatography ID: | CH004018 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) |
| Column Temperature: | 25 |
| Flow Gradient: | 0-3min：70%A:30%B，3-20min：40%A:60%B, 20-27min：20%A:80%B，27-30min：70%A;30%B |
| Flow Rate: | 0.25mL/min |
| Solvent A: | 100% water; 0.3% ethanoic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS005044 |
| Analysis ID: | AN005314 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Arachidonic acid metabolites were quantified by use of a 5500 QTRAP hybrid triple quadrupole linear ion trap mass spectrometer (AB Sciex, Foster City, CA, USA) equipped with a Turbo Ion Spray electrospray ionization source. The mass spectrometer was operated using the software Analyst 1.5.1. Analytes were detected using multiple reaction monitoring (MRM) scans in negative mode. The dwell time used for all MRM experiments was 25 ms. The ion source parameters were set as follows: CUR = 40 psi, GS1 = 30 psi, GS2 = 30 psi, IS = −4500 V, CAD = medium, and temp = 500°C. Metaboanalyst 3.0 (http://www.metaboanalyst.ca) was used for metabolomic data analysis. |
| Ion Mode: | NEGATIVE |
