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MB Sample ID: SA354253
| Local Sample ID: | Serum_2 |
| Subject ID: | SU003384 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003384 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Serum_2 | SA354253 | FL041339 | Control | Genoptye |
| Serum_2 | SA354253 | FL041339 | Serum | Sample source |
Collection:
| Collection ID: | CO003377 |
| Collection Summary: | Standard chow fed mice were fasted for four hours prior to serum or tissue collection. |
| Sample Type: | Serum |
Treatment:
| Treatment ID: | TR003393 |
| Treatment Summary: | Samples are from wild-type or SLC25A48-KO male mice fed a standard chow diet. Mice were fasted for four hours prior to sample collection. |
Sample Preparation:
| Sampleprep ID: | SP003391 |
| Sampleprep Summary: | 10 µL serum was mixed with 90 µL extraction solvent of methanol: acetonitrile: water 40: 40: 20 at -20 ˚C, and then vigorously vortexed. All samples were then conditioned to -4 ˚C, and centrifuged at 16,000 rpm/min. The supernatant was transferred to LC-MS tubes for analysis. |
Combined analysis:
| Analysis ID | AN005348 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | Waters XBridge BEH Amide XP (150 x 2.1mm, 2.5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF |
| Ion Mode | UNSPECIFIED |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH004050 |
| Chromatography Summary: | Waters XBridge BEH Amide XP Column (150 x 2.1mm, 2.5um) with guard column (5mm x 2.1mm, 2.5 µm) |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters XBridge BEH Amide XP (150 x 2.1mm, 2.5um) |
| Column Temperature: | 4˚C |
| Flow Gradient: | 0 - 3 min, 100% B; 3.2 - 6.2 min, 90% B; 6.5 - 10.5 min, 80% B; 10.7 - 13.5 min, 70% B; 13.7 - 16 min, 45% B; and 16.5 - 22 min, 100% B |
| Flow Rate: | 0.3 mL/ min |
| Solvent A: | 95% Water, 5% Acetonitrile; 10 mM ammonium acetate and 10 mM ammonium hydroxide |
| Solvent B: | 20% Water, 80% Acetonitrile; 10 mM ammonium acetate and 10 mM ammonium hydroxide |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS005078 |
| Analysis ID: | AN005348 |
| Instrument Name: | Thermo Q Exactive HF |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The mass spectrometry used was Q Exactive HF (Thermo Fisher Scientific, San Jose, CA), and scanned from 70 to 1000 m/z with switching polarity. The resolution was 120,000. Metabolites were identified based on accurate mass and retention time using an in-house library, and the software used was EI-Maven (Elucidata, Cambridge, MA). |
| Ion Mode: | UNSPECIFIED |
