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MB Sample ID: SA354256
Local Sample ID: | Serum_6 |
Subject ID: | SU003384 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003384 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Serum_6 | SA354256 | FL041339 | Control | Genoptye |
Serum_6 | SA354256 | FL041339 | Serum | Sample source |
Collection:
Collection ID: | CO003377 |
Collection Summary: | Standard chow fed mice were fasted for four hours prior to serum or tissue collection. |
Sample Type: | Serum |
Treatment:
Treatment ID: | TR003393 |
Treatment Summary: | Samples are from wild-type or SLC25A48-KO male mice fed a standard chow diet. Mice were fasted for four hours prior to sample collection. |
Sample Preparation:
Sampleprep ID: | SP003391 |
Sampleprep Summary: | 10 µL serum was mixed with 90 µL extraction solvent of methanol: acetonitrile: water 40: 40: 20 at -20 ˚C, and then vigorously vortexed. All samples were then conditioned to -4 ˚C, and centrifuged at 16,000 rpm/min. The supernatant was transferred to LC-MS tubes for analysis. |
Combined analysis:
Analysis ID | AN005348 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | Waters XBridge BEH Amide XP (150 x 2.1mm, 2.5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive HF |
Ion Mode | UNSPECIFIED |
Units | Peak area |
Chromatography:
Chromatography ID: | CH004050 |
Chromatography Summary: | Waters XBridge BEH Amide XP Column (150 x 2.1mm, 2.5um) with guard column (5mm x 2.1mm, 2.5 µm) |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters XBridge BEH Amide XP (150 x 2.1mm, 2.5um) |
Column Temperature: | 4˚C |
Flow Gradient: | 0 - 3 min, 100% B; 3.2 - 6.2 min, 90% B; 6.5 - 10.5 min, 80% B; 10.7 - 13.5 min, 70% B; 13.7 - 16 min, 45% B; and 16.5 - 22 min, 100% B |
Flow Rate: | 0.3 mL/ min |
Solvent A: | 95% Water, 5% Acetonitrile; 10 mM ammonium acetate and 10 mM ammonium hydroxide |
Solvent B: | 20% Water, 80% Acetonitrile; 10 mM ammonium acetate and 10 mM ammonium hydroxide |
Chromatography Type: | HILIC |
MS:
MS ID: | MS005078 |
Analysis ID: | AN005348 |
Instrument Name: | Thermo Q Exactive HF |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The mass spectrometry used was Q Exactive HF (Thermo Fisher Scientific, San Jose, CA), and scanned from 70 to 1000 m/z with switching polarity. The resolution was 120,000. Metabolites were identified based on accurate mass and retention time using an in-house library, and the software used was EI-Maven (Elucidata, Cambridge, MA). |
Ion Mode: | UNSPECIFIED |