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MB Sample ID: SA358665
| Local Sample ID: | T3_4_pos |
| Subject ID: | SU003428 |
| Subject Type: | Soil sample |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003428 |
| Subject Type: | Soil sample |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| T3_4_pos | SA358665 | FL041871 | 0.1 mg/L | endo-borneol concentration |
| T3_4_pos | SA358665 | FL041871 | soil | Sample source |
Collection:
| Collection ID: | CO003421 |
| Collection Summary: | Rhizosphere soil samples were collected from Panax ginseng that were leaf sprayed with endo-borneol at 0, 0.1, 100 mg/L. The samples were snap-frozen using liquid nitrogen and stored in -80°C for metabolism analysis. |
| Sample Type: | Soil |
| Collection Method: | Gently brushed from the roots of Panax ginseng |
| Collection Location: | Laobiangou, County Benxi, Liaoning, China |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003437 |
| Treatment Summary: | Panax ginseng were leaf sprayed with endo-borneol at 0, 0.1, 100 mg/L from July to August, 2021. Six replicates were set for each group as six plots.Then the rhizosphere soil of Panax ginseng from each treatment was collected. |
Sample Preparation:
| Sampleprep ID: | SP003435 |
| Sampleprep Summary: | 50 mg solid sample was added to a 2 mL centrifuge tube and a 6 mm diameter grinding bead was added. 400 μL of extraction solution (methanol: water = 4:1 (v:v)) containing 0.02 mg/mL of internal standard (L-2-chlorophenylalanine) was used for metabolite extraction. Samples were ground by the Wonbio-96c (Shanghai wanbo biotechnology co., ltd) frozen tissue grinder for 6 min (-10°C, 50 Hz), followed by low-temperature ultrasonic extraction for 30 min (5°C, 40 kHz). The samples were left at -20°C for 30 min, centrifuged for 15 min (4°C, 13000 g), and the supernatant was transferred to the injection vial for LC-MS/MS analysis. |
Combined analysis:
| Analysis ID | AN005418 | AN005419 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish Horizon | Thermo Vanquish Horizon |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF-X Orbitrap | Thermo Q Exactive HF-X Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH004108 |
| Instrument Name: | Thermo Vanquish Horizon |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 40℃ |
| Flow Gradient: | 0-3 min: 0-20% B, 3-9 min: 20-60% B, 9-11 min: 60-100% B, maintain for 2.5 min, 13.5-13.6 min: 100% to 0% B, maintain for 2.4 min |
| Flow Rate: | 0.40 mL/min |
| Solvent A: | 95% water/5% acetonitrile; 0.1% formic acid |
| Solvent B: | 47.5% acetonitrile/47.5% isopropanol/5% water; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS005144 |
| Analysis ID: | AN005418 |
| Instrument Name: | Thermo Q Exactive HF-X Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Full MS resolution was 60000, and MS/MS resolution was 7500. Data acquisition was performed with the Data Dependent Acquisition (DDA) mode. The detection was carried out over a mass range of 70-1050 m/z. |
| Ion Mode: | POSITIVE |
| MS ID: | MS005145 |
| Analysis ID: | AN005419 |
| Instrument Name: | Thermo Q Exactive HF-X Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Full MS resolution was 60000, and MS/MS resolution was 7500. Data acquisition was performed with the Data Dependent Acquisition (DDA) mode. The detection was carried out over a mass range of 70-1050 m/z. |
| Ion Mode: | NEGATIVE |
