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MB Sample ID: SA358843
| Local Sample ID: | May_2 |
| Subject ID: | SU003430 |
| Subject Type: | Plant |
| Subject Species: | Pinus koraiensis |
| Taxonomy ID: | 88728 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003430 |
| Subject Type: | Plant |
| Subject Species: | Pinus koraiensis |
| Taxonomy ID: | 88728 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| May_2 | SA358843 | FL041893 | May | Month |
| May_2 | SA358843 | FL041893 | Pinus koraiensis leaf | Sample source |
| May_2 | SA358843 | FL041893 | May | Month |
| May_2 | SA358843 | FL041893 | Pinus koraiensis leaf | Sample source |
Collection:
| Collection ID: | CO003423 |
| Collection Summary: | Fresh pine needles were collected from forests of Pinus koraiensis located in Benxi (41°4′17″ N, 124°29′58″ E, 578 m, 965.9 hPa) , Liaoning province, China. From May to September, 2020, fresh needles were collected on a monthly basis, and brought back to laboratory to prepare needle leachates. Every 150 g of fresh needles collected from Pinus koraiensis on a monthly basis during May to September were soaked in 1000 ml of sterilized water for 48 hours at room temperature, and subsequently filtered twice using qualitative filter paper through suction filtration. The aqueous leachates with a concentration of 150 g/L were stored at 4 °C for further analysis. |
| Sample Type: | Leaf |
Treatment:
| Treatment ID: | TR003439 |
| Treatment Summary: | No treatment |
Sample Preparation:
| Sampleprep ID: | SP003437 |
| Sampleprep Summary: | A volume of 100 ml of leachates was extracted by 300 ml ethyl acetate (Analytical grade, Tianjin Fengchuan Chemical Reagent Co., Ltd, 426 Tianjin, China) then evaporated by rotary evaporator at 45°C with 4°C-condensed water recycled in low-temperature circulator. The remaining sediment was 429 rinsed with 2.5 ml of hexane (HPLC, EMD Millipore, Billerica, MA, USA). The liquid hexane was filtered using a 0.22 um Millipore filter (Catridge filter, Jin Teng, China) for gas chromatography-mass spectrometry (GC-MS) analysis. |
Combined analysis:
| Analysis ID | AN005421 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Shimadzu GCMS-QP2010 ultra |
| Column | Shimadzu SH-Rxi-5Sil MS (30m x 0.25mm x 0.25um) |
| MS Type | EI |
| MS instrument type | GC-MS |
| MS instrument name | Shimadzu QP2010 Ultra |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH004110 |
| Chromatography Summary: | The temperature program of GC-MS started from an initial temperature of 40 ℃, followed by an increase of 3 ℃/min to 80 ℃ and then an increase of 5 ℃/min from 80 ℃ to 260 ℃, which was maintained for 30 min. |
| Instrument Name: | Shimadzu GCMS-QP2010 ultra |
| Column Name: | Shimadzu SH-Rxi-5Sil MS (30m x 0.25mm x 0.25um) |
| Column Temperature: | 40 - 260 |
| Flow Gradient: | Not applicable |
| Flow Rate: | Not applicable |
| Injection Temperature: | 250 ℃ |
| Solvent A: | Not applicable |
| Solvent B: | Not applicable |
| Analytical Time: | 79.33 min |
| Chromatography Type: | GC |
MS:
| MS ID: | MS005147 |
| Analysis ID: | AN005421 |
| Instrument Name: | Shimadzu QP2010 Ultra |
| Instrument Type: | GC-MS |
| MS Type: | EI |
| MS Comments: | The mass spectrum was recorded for the mass range 35–500 m/z and scanned at a rate of 0.3s. All the compounds identified by GCMS solutions software (Shimadzu, Kyoto, Japan) based on the comparison of its mass spectra (MS) with the NIST14 (National Institute of Standards and Technology) library. |
| Ion Mode: | POSITIVE |
