Return to study ST003310 main page
MB Sample ID: SA358859
| Local Sample ID: | H4 |
| Subject ID: | SU003431 |
| Subject Type: | Plant |
| Subject Species: | Pinus koraiensis |
| Taxonomy ID: | 88728 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003431 |
| Subject Type: | Plant |
| Subject Species: | Pinus koraiensis |
| Taxonomy ID: | 88728 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| H4 | SA358859 | FL041901 | high level | Precipitation |
| H4 | SA358859 | FL041901 | Pinus koraiensis leaf leachate | Sample source |
Collection:
| Collection ID: | CO003424 |
| Collection Summary: | Based on historical meteorological data, monthly precipitations were graded as low (34.6 mm, 771.63 min), medium (104.2 mm, 2223.07 min) and high (285.8 mm, 5233.03 min) level. To determine the representative weight of fresh needles per unit area in the forest of P. koraiensis, the tree canopy aboveground within one square meter was trimmed, and the fresh needles therein were weighed at five random points. The average weight (3980.58 g/m2) was then established as the representative weight of fresh needles per square meter. A weight of 84.03 g fresh needles were evenly laid on 2-layers absorbent gauze and fixed at the top of a 5-liter vessel (16.4 cm inside diameter), which was placed on the ground and filled with certain volume of double distilled water for simulated graded rainfall based on graded monthly precipitation. A peristaltic pump with a constant flow rate of 3770 ml/min was used to introduce the water to percolate through the packed fresh needle surface. The simulated leachates were filtered twice with qualitative filter paper via suction filtration and stored at 4 °C for quantification. |
| Sample Type: | Leaf leachate |
Treatment:
| Treatment ID: | TR003440 |
| Treatment Summary: | A volume of 100 ml of leachates was extracted by 300 ml ethyl acetate then evaporated by rotary evaporator at 45°C with 4°C-condensed water recycled in low-temperature circulator. The remaining sediment was rinsed with 2.5 ml of hexane. The liquid hexane was filtered using a 0.22 um Millipore filter for further analysis. |
Sample Preparation:
| Sampleprep ID: | SP003438 |
| Sampleprep Summary: | After rotary evaporation, the remaining sediment was washed out with 5 ml of ethyl acetate. The ethyl acetate was then filtered with a 0.22 um Millipore filter for analysis. A series concentration of endo-borneol standard were formulated in ethyl acetate to create a standard curve. |
Combined analysis:
| Analysis ID | AN005422 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 8860 GC |
| Column | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| MS Type | EI |
| MS instrument type | GC/MSD |
| MS instrument name | Agilent 5977B |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH004111 |
| Chromatography Summary: | The temperature program started from an initial temperature of 50 ℃, followed by an increase of 12℃/min to 160 ℃. |
| Instrument Name: | Agilent 8860 GC |
| Column Name: | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| Column Temperature: | 50 - 160 |
| Flow Gradient: | Not applicable |
| Flow Rate: | Not applicable |
| Injection Temperature: | 200 ℃ |
| Solvent A: | Not applicable |
| Solvent B: | Not applicable |
| Chromatography Type: | GC |
MS:
| MS ID: | MS005148 |
| Analysis ID: | AN005422 |
| Instrument Name: | Agilent 5977B |
| Instrument Type: | GC/MSD |
| MS Type: | EI |
| MS Comments: | An electron ionization system with ionization energy of 7 eV was used. Helium gas (99.999%) was used as the carrier gas at constant flow rate 1 ml/min and an injection volume of 1 ul was employed. The ion-source temperature was 230 °C. The scan interval of mass spectra was 3.1 units/s and fragments from 35 to 270 da. Afterwards, Agilent MassHunter Workstation Quantitative Analysis B.07.01 were used as analytical software. The mass spectrum of the individual peak was inspected manually to confirm the peak had the correct m/z diagnostic fragments, matched with the search results from NIST MS Search 2.2 library. For endo-borneol, the target ion is m/z 95, and qualifier ions are m/z 110 and m/z 67. |
| Ion Mode: | POSITIVE |
