Summary of Study ST003265

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002027. The data can be accessed directly via it's Project DOI: 10.21228/M8M24P This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003265
Study TitleMalic Enzyme 2 maintains metabolic state and anti-tumor immunity of CD8+ T cells
Study SummaryFor metabolite analysis, naive ME2+/+ and ME2-/- CD8+ T cells were isolated and activated with anti-CD3 (4μg/ml) and anti-CD28 (1μg/ml). Cells were washed twice with ice-cold PBS and metabolites were extracted with ice-cold 80% methanol. The extracts were analyzed by LC-MS/MS.
Institute
Peking Union Medical College
Last Namezhang
First Namezhenxi
AddressBeijing Dongdan Santiao No.5
Email13121716766@163.com
Phone69156953
Submit Date2024-06-05
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-07-15
Release Version1
zhenxi zhang zhenxi zhang
https://dx.doi.org/10.21228/M8M24P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN005349
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Dionex Ultimate 3000
Column Merck SeQuant ZIC-HILIC (150 x 2.1mm,5um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Focus
Ion Mode UNSPECIFIED
Units Peak area
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