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MB Sample ID: SA003619
Local Sample ID: | LI_8 39_POS_RF |
Subject ID: | SU000088 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | approx. 15-16 weeks |
Gender: | male |
Animal Animal Supplier: | The Jackson Laboratory |
Animal Feed: | high fat, low fat, or chow |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000107 | AN000108 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Waters Acquity | Waters Acquity |
Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Waters Synapt G2 | Waters Synapt G2 |
Ion Mode | POSITIVE | NEGATIVE |
Units | Normalized abundance | Normalized abundance |
Chromatography:
Chromatography ID: | CH000075 |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
Column Pressure: | 6000-10000 |
Column Temperature: | 50 C |
Flow Gradient: | Time(min) Flow Rate %A %B Curve ; 1. Initial 0.400 100.0 0.0 ; 2. 1.00 0.400 100.0 0.0 6 ; 3. 16.00 0.400 0.0 100.0 6 ; 4. 20.00 0.400 0.0 100.0 6 ; 5. 22.00 0.400 100.0 0.0 6 |
Flow Rate: | 0.4 mL/min |
Injection Temperature: | 8 C |
Internal Standard: | L-Tryptophan-d5 |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% methanol; 0.1% formic acid |
Analytical Time: | 22 min |
Weak Wash Solvent Name: | 5%MeOH |
Weak Wash Volume: | 1000 uL |
Strong Wash Solvent Name: | 80%MeOH |
Strong Wash Volume: | 1000 uL |
Target Sample Temperature: | 8 C |
Sample Loop Size: | 10 uL |
Sample Syringe Size: | 100 uL |
Randomization Order: | Yes |
Chromatography Type: | Reversed phase |