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MB Sample ID: SA005551
Local Sample ID: | 6PHS-95 |
Subject ID: | SU000119 |
Subject Type: | Invertebrate |
Subject Species: | Caenorhabditis elegans |
Taxonomy ID: | 6239 |
Genotype Strain: | N2 |
Age Or Age Range: | Young adult |
Gender: | Hermaphrodite |
Species Group: | Invertebrate |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000165 | AN000166 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units |
Chromatography:
Chromatography ID: | CH000114 |
Chromatography Summary: | Samples were analyzed using a mass range of m/z 70-1000 in positive and negative ionization mode, externally calibrated, using a Thermo Scientific Q-Exactive Orbitrap mass spectrometer equipped with a Dionex UltiMate 3000 RS autosampler and pump. Three ?L of each sample were injected onto an Acquity UPLC HSS T3 (2.1x100 mm, 1.8 micron) column using a column temperature of 35 C and a flow rate of 300 L/min with solvent A (0.1% formic acid in water (Fisher LC-MS Optima)) held at 100% A for 1 min, followed by linear increase to 80% B (0.1% formic acid in acetonitrile) over 10 min, kept constant for 2 min, followed by a return to initial conditions over 0.5 min and equilibration for 3 min. |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
Flow Gradient: | A held at 100% A for 1 min, followed by linear increase to 80% B over 10 min, kept constant for 2 min, followed by a return to initial conditions over 0.5 min and equilibration for 3 min |
Flow Rate: | 300 L/min |
Sample Injection: | 3 ?L |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |