Return to study ST000163 main page
MB Sample ID: SA008764
Local Sample ID: | VT_130304_108 |
Subject ID: | SU000182 |
Subject Type: | Human/animal |
Subject Species: | Homo sapiens;Callithrix jacchus |
Taxonomy ID: | 9606;9483 |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000255 |
---|---|
Analysis type | MS |
Chromatography type | Anion exchange/Reversed phase |
Chromatography system | Thermo Accela CTC |
Column | Higgins C18 (100 x 2.1mm,5um); Hamilton Anion Exchange 100x2.1mm,7um |
MS Type | ESI |
MS instrument type | Single quadrupole |
MS instrument name | Thermo LTQ |
Ion Mode | POSITIVE |
Units | Intensity |
Chromatography:
Chromatography ID: | CH000180 |
Chromatography Summary: | untargeted dual chromatography (C18 and Anion Exchange column) |
Methods ID: | Methods_LTQVelosOrbi |
Methods Filename: | Methods_LTQVelosOrbi |
Instrument Name: | Thermo Accela CTC |
Column Name: | Higgins C18 (100 x 2.1mm,5um); Hamilton Anion Exchange 100x2.1mm,7um |
Column Pressure: | 200 Bar (max) |
Column Temperature: | 30 |
Flow Gradient: | C18: A% = 5% for 10 minutes, 0-2mins = 60% B, 2-6mins = gradient to 95% B, 6-10mins = 95% B; AE: 1-10mins = 50%B, 0-2mins = 5%A, 2-7mins = gradient to 50%A, 7-10mins = 50%A |
Flow Rate: | 0.35 mL/min |
Injection Temperature: | 4C |
Sample Injection: | 10ul |
Sampling Cone: | ESI |
Solvent A: | 100% water; 0.2% formic acid |
Solvent B: | 100% acetonitrile |
Analytical Time: | LC grade water |
Capillary Voltage: | 10 minutes |
Migration Time: | 100uA (source current) |
Sheath Liquid: | 4.5kV (source voltage) |
Weak Wash Solvent Name: | water with 10% methanol |
Sample Syringe Size: | 20ul |
Chromatography Type: | Anion exchange/Reversed phase |