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MB Sample ID: SA008896
| Local Sample ID: | VT_120218_020 |
| Subject ID: | SU000182 |
| Subject Type: | Human/animal |
| Subject Species: | Homo sapiens;Callithrix jacchus |
| Taxonomy ID: | 9606;9483 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000255 |
|---|---|
| Analysis type | MS |
| Chromatography type | Anion exchange/Reversed phase |
| Chromatography system | Thermo Accela CTC |
| Column | Higgins C18 (100 x 2.1mm,5um); Hamilton Anion Exchange 100x2.1mm,7um |
| MS Type | ESI |
| MS instrument type | Single quadrupole |
| MS instrument name | Thermo LTQ |
| Ion Mode | POSITIVE |
| Units | Intensity |
Chromatography:
| Chromatography ID: | CH000180 |
| Chromatography Summary: | untargeted dual chromatography (C18 and Anion Exchange column) |
| Methods ID: | Methods_LTQVelosOrbi |
| Methods Filename: | Methods_LTQVelosOrbi |
| Instrument Name: | Thermo Accela CTC |
| Column Name: | Higgins C18 (100 x 2.1mm,5um); Hamilton Anion Exchange 100x2.1mm,7um |
| Column Pressure: | 200 Bar (max) |
| Column Temperature: | 30 |
| Flow Gradient: | C18: A% = 5% for 10 minutes, 0-2mins = 60% B, 2-6mins = gradient to 95% B, 6-10mins = 95% B; AE: 1-10mins = 50%B, 0-2mins = 5%A, 2-7mins = gradient to 50%A, 7-10mins = 50%A |
| Flow Rate: | 0.35 mL/min |
| Injection Temperature: | 4C |
| Sample Injection: | 10ul |
| Sampling Cone: | ESI |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | LC grade water |
| Capillary Voltage: | 10 minutes |
| Migration Time: | 100uA (source current) |
| Sheath Liquid: | 4.5kV (source voltage) |
| Weak Wash Solvent Name: | water with 10% methanol |
| Sample Syringe Size: | 20ul |
| Chromatography Type: | Anion exchange/Reversed phase |
