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MB Sample ID: SA009300
Local Sample ID: | ms2418-25 |
Subject ID: | SU000187 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
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Combined analysis:
Analysis ID | AN000262 |
---|---|
Analysis type | MS |
Chromatography type | |
Chromatography system | Waters Acquity |
Column | |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Thermo Quantum Ultra |
Ion Mode | POSITIVE |
Units | uM |
Chromatography:
Chromatography ID: | CH000185 |
Chromatography Summary: | Amino acids in particular are relatively stable for several years, with the exception of their keto-acids (e.g., KIC), which we have not included in the present analysis. Subsequently, we separated the samples using an Acquity UPLC system, followed by mass detection using a TSQ Ultra Quantum from Thermo Finnigan running in ESI positive mode as previously described [32]. We calculated the AA and AA metabolites concentrations using 10 point standard curves as previously described [32]. We handled the pre- and post-intervention samples similarly and analyzed them in the same run. The reproducibility of the UPLC/MS/MS method in the MCRMC is excellent (average CV ~ 9.5%) [32]. |
Instrument Name: | Waters Acquity |