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MB Sample ID: SA010730
| Local Sample ID: | SF-100109-199546 |
| Subject ID: | SU000239 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | Moffitt Cancer Center Tissue Core |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000325 | AN000326 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity |
| Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Waters Synapt G2 Si QTOF | Waters Synapt G2 Si QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Normalized abundance | Normalized abundance |
Chromatography:
| Chromatography ID: | CH000245 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| Column Pressure: | 6000-10000 |
| Column Temperature: | 50 C |
| Flow Gradient: | Time(min) Flow Rate %A %B Curve; 1. Initial 0.400 100.0 0.0; 2. 1.00 0.400 100.0 0.0 6;3. 16.00 0.400 0.0 100.0 6; 4. 20.00 0.400 0.0 100.0 6; 5. 22.00 0.400 100.0 0.0 6 |
| Flow Rate: | 0.4 mL/min |
| Injection Temperature: | 8 C |
| Internal Standard: | L-Tryptophan-d5 |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Analytical Time: | 22 min |
| Weak Wash Solvent Name: | 5%MeOH |
| Weak Wash Volume: | 1000 uL |
| Strong Wash Solvent Name: | 80%MeOH |
| Strong Wash Volume: | 1000 uL |
| Target Sample Temperature: | 8 C |
| Sample Loop Size: | 10 uL |
| Sample Syringe Size: | 100 uL |
| Randomization Order: | Yes |
| Chromatography Type: | Reversed phase |
