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MB Sample ID: SA011605
| Local Sample ID: | P25 |
| Subject ID: | SU000274 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000402 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex API 4000 QTrap |
| Ion Mode | NEGATIVE |
| Units |
Chromatography:
| Chromatography ID: | CH000284 |
| Chromatography Summary: | Reversed-Phase Gradient Seperation |
| Methods ID: | RTI-RCMRC-IPA-Analysis |
| Methods Filename: | RTI-RCMRC-IPA-Analysis |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| Column Pressure: | 6000-10000 |
| Column Temperature: | 50 C |
| Flow Gradient: | Time(min) Flow Rate %A %B Curve ;Initial 0.600 99.0 1.0 6;0.75 0.600 99.0 1.0 6 ;2.75 0.600 1.0 99.0 6 ;3.00 0.600 1.0 99.0 6 ;3.10 0.600 99.0 1.0 6 ;3.50 0.600 99.0 1.0 6; |
| Flow Rate: | 0.6 ml/min |
| Injection Temperature: | 8 C |
| Internal Standard: | 2-methyl-4-chlorophenyl-acetic acid (MCPA) |
| Solvent A: | 95% water/5% acetonitrile; 0.15% acetic acid |
| Solvent B: | 95% acetonitrile/5% water; 0.15% acetic acid |
| Analytical Time: | 3.5 mins |
| Weak Wash Solvent Name: | 95:5 Water:ACN with 0.15% Acetic Acid |
| Weak Wash Volume: | 600 uL |
| Strong Wash Solvent Name: | 95:5 ACN:Water with 0.15% Acetic Acid |
| Strong Wash Volume: | 600 uL |
| Target Sample Temperature: | 8 C |
| Sample Loop Size: | 10 uL |
| Sample Syringe Size: | 100 uL |
| Randomization Order: | Yes |
| Chromatography Type: | Reversed phase |
