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MB Sample ID: SA011753
Local Sample ID: | SW Hypo 3 |
Subject ID: | SU000277 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | 30 weeks |
Gender: | Female |
Animal Housing: | Singly housed |
Animal Feed: | High fat diet (D12492, Research Diets, New Brunswick, NJ), Low fat diet (D12450, Research Diets, New Brunswick, NJ) |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000406 | AN000407 | AN000408 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | |||
Chromatography system | Waters Acquity | Waters Acquity | Waters Acquity |
Column | |||
MS Type | ESI | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex API 4000 QTrap | ABI Sciex API 4000 QTrap | ABI Sciex API 4000 QTrap |
Ion Mode | NEGATIVE | POSITIVE | POSITIVE |
Units | % Area Ratio | nM | nM |
Chromatography:
Chromatography ID: | CH000287 |
Chromatography Summary: | Oxylipin analysis |
Methods Filename: | NewmanLab-Mediators-Protocol-PT.pdf |
Instrument Name: | Waters Acquity |
Column Temperature: | 60 C |
Flow Gradient: | See protocol/methods file |
Flow Rate: | 0.25 |
Internal Standard: | See protocol/methods file |
Retention Time: | See protocol/methods file |
Sample Injection: | 5 L |
Solvent A: | 100% water; 0.1% acetic acid |
Solvent B: | 90% acetonitrile/ 10% isopropanol |
Analytical Time: | 16 min |
Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
Weak Wash Volume: | 600 L |
Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
Strong Wash Volume: | 600 L |
Sample Loop Size: | 17 L |
Chromatography ID: | CH000288 |
Chromatography Summary: | Endocannabinoid analysis |
Methods Filename: | NewmanLab-Mediators-Protocol-PT.pdf |
Instrument Name: | Waters Acquity |
Column Temperature: | 60 C |
Flow Gradient: | See protocol/methods file |
Flow Rate: | 0.25 |
Internal Standard: | See protocol/methods file |
Retention Time: | See protocol/methods file |
Sample Injection: | 5 L |
Solvent A: | 100% water; 0.1% acetic acid |
Solvent B: | 90% acetonitrile/ 10% isopropanol |
Analytical Time: | 11 min |
Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
Weak Wash Volume: | 600 L |
Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
Strong Wash Volume: | 600 L |
Sample Loop Size: | 17 L |
Chromatography ID: | CH000289 |
Chromatography Summary: | Ceramide analysis |
Methods Filename: | NewmanLab-Mediators-Protocol-PT.pdf |
Instrument Name: | Waters Acquity |
Column Temperature: | 60 C |
Flow Gradient: | See protocol/methods file |
Flow Rate: | 0.25 |
Internal Standard: | See protocol/methods file |
Retention Time: | See protocol/methods file |
Sample Injection: | 5 L |
Solvent A: | 100% water; 5 mM ammonium formate; 0.2% formic acid |
Solvent B: | 100% methanol; 0.2% formic acid; 5 mM ammonium formate |
Analytical Time: | 15 min |
Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
Weak Wash Volume: | 600 L |
Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
Strong Wash Volume: | 600 L |
Sample Loop Size: | 17 L |