Return to study ST000269 main page
MB Sample ID: SA012112
Local Sample ID: | QE1_REP_18_25 |
Subject ID: | SU000289 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Age Or Age Range: | 8 or 24 week |
Gender: | male |
Animal Animal Supplier: | Jackson Laboratories |
Animal Feed: | Control (Research Diets, Inc. # D09100304) and trans-fat diet (Research Diets, Inc. # D09100301) |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000431 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | nmol/g liver protein |
Chromatography:
Chromatography ID: | CH000301 |
Chromatography Summary: | Waters Acquity BEH C18 (2.1 x 50 mm, 1.7 um) with guard was used to separate compounds with mobile phases made of isopropanol, acetonitrile, and water. |
Methods Filename: | REP_18 |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
Column Temperature: | 30 C |
Flow Gradient: | 32% B to 98% B over 18 mins |
Flow Rate: | 0.5 mL/min |
Injection Temperature: | 4 C |
Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
Analytical Time: | 18 mins |
Capillary Voltage: | 350 C |
Time Program: | 0-1 min: 32%B --> 1-1.5 min: 40% B --> 4 min: 45% B --> 5 min: 50% B --> 8 min: 60% B --> 11 min: 70% B --> 14 min: 80% B --> 16-17 min: 100%B --> 18 min: 32%B --> 21 min: 32% B |
Strong Wash Solvent Name: | 1:1:1 isopropanol:acetonitrile:water |
Strong Wash Volume: | 100 uL |
Chromatography Type: | Reversed phase |