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MB Sample ID: SA013100
Local Sample ID: | 1_2 |
Subject ID: | SU000306 |
Subject Type: | Animal cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57/Bl6 |
Subject Comments: | Primary mouse myocytes were used for this study. The cells were obtained from gastrocnemius muscle of male 8 weeks-old C57BL/6J mice. |
Species Group: | Mammal |
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Combined analysis:
Analysis ID | AN000454 | AN000455 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Scientific-Dionex Ultimate 3000: | Thermo Scientific-Dionex Ultimate 3000: |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak height | peak height |
Chromatography:
Chromatography ID: | CH000324 |
Chromatography Comments: | UHPLC, Thermo Scientific-Dionex Ultimate 3000: While setting up sequence, ensure that method to be utilized is PFP-metabolomics-neg350-NoBuffer-0-SID and PFP-metabolomics-neg-350-dd-MS2 for negative sequences. For positive sequences utilize PFP-metabolomics-pos-350-0-SID and PFP-metabolomics-pos-350-dd-MS2. Check the lines for air bubbles and purge line if present. Set injection volume to 2uL for positive samples and 4uL for negative samples. |
Instrument Name: | Thermo Scientific-Dionex Ultimate 3000: |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Chromatography Type: | Reversed phase |