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MB Sample ID: SA015061
Local Sample ID: | 115342 |
Subject ID: | SU000357 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | Not applicable |
Age Or Age Range: | Variable |
Weight Or Weight Range: | Variable |
Height Or Height Range: | Variable |
Gender: | Variable |
Human Race: | Variable |
Human Ethnicity: | Variable |
Human Trial Type: | Not applicable |
Human Lifestyle Factors: | Not applicable |
Human Medications: | Not applicable |
Human Prescription Otc: | Not applicable |
Human Smoking Status: | Not applicable |
Human Alcohol Drug Use: | Not applicable |
Human Nutrition: | Not applicable |
Human Inclusion Criteria: | Deceased organ donor with consent for use of tissue for research |
Human Exclusion Criteria: | None |
Animal Animal Supplier: | in house/in house |
Subject Comments: | Liver biopsy is routinely performed on livers used for solid organ transplantation. The tissue is from decedent subjects. Consent for use of tissue is obtained at the time of organ donation by the local organ procurement organization. |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000544 | AN000545 | AN000546 | AN000547 | AN000548 |
---|---|---|---|---|---|
Analysis type | MS | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity | Thermo Dionex Ultimate 3000 |
Column | Waters AccQ Tag (100 x 2mm,1.7um) | Waters Acquity BEH C18 (50 x 2mm,1.7um) | Thermo Hypercarb (50 x 2.1mm,3um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
MS Type | ESI | ESI | ESI | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ | Agilent 6490 QQQ | Agilent 6490 QQQ | Agilent 6490 QQQ | Thermo Quantiva QQQ |
Ion Mode | POSITIVE | POSITIVE | POSITIVE | POSITIVE | POSITIVE |
Units | nmol/mg dry powder | pmol/mg dry powder | nmol/mg dry powder | nmol/mg dry powder | nmol/mg dry powder |
Chromatography:
Chromatography ID: | CH000381 |
Chromatography Summary: | Amino acid assay |
Methods Filename: | SB_AA_Assay.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
Column Temperature: | 55 |
Flow Rate: | 0.7 mL/min |
Solvent A: | AccQ·Tag Eluent A |
Solvent B: | accQ·Tag Eluent B |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000382 |
Chromatography Summary: | CoA assay |
Methods Filename: | SB_CoA_Assay.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters Acquity BEH C18 (50 x 2mm,1.7um) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000383 |
Chromatography Summary: | NAD-ATP assay |
Methods Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Thermo Hypercarb (50 x 2.1mm,3um) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000384 |
Chromatography Summary: | NADH assay |
Methods Filename: | SB_NADH_Assay_Agilent.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
Column Temperature: | 30 |
Flow Rate: | 0.54 mL/min |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 6 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000385 |
Chromatography Summary: | Organic acid assay |
Methods Filename: | SB_OA_Assay.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
Column Temperature: | 45 |
Flow Rate: | 0.3 mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |