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MB Sample ID: SA024743

Local Sample ID:MS5035_6_120
Subject ID:SU000506
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

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Combined analysis:

Analysis ID AN000751
Analysis type MS
Chromatography type GC
Chromatography system Agilent
Column Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5973
Ion Mode POSITIVE
Units mole percent enrichment

Chromatography:

Chromatography ID:CH000539
Chromatography Summary:Glucose concentration was measured every 10 min during the insulin clamp with an Analox glucose analyzer (Analox Instruments, London, U.K.). [6,6-2H2]-d-glucose enrichment in the plasma and infusate was measured using gas chromatography–mass spectrometry. As described previously, the steady-state equations of Steele et al. (29) were used to calculate the rate of glucose appearance (Ra) and disappearance (Rd). EGP was calculated as the difference between total glucose Ra and the exogenous glucose infusion rate, peripheral insulin sensitivity was assessed from the rate of glucose infusion required to maintain euglycemia during the high-dose insulin clamp, and hepatic insulin sensitivity was assessed by the extent to which EGP was suppressed from baseline to low-dose hyperinsulinemia (26).
Instrument Name:Agilent
Column Name:Agilent DB5-MS (30m × 0.25mm, 0.25um)
Chromatography Type:GC
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