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MB Sample ID: SA027819
Local Sample ID: | S_77 |
Subject ID: | SU000552 |
Subject Type: | Serum |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BLKsJ-db/db (disease model) and C57BLKsJ wild type (wild type) |
Age Or Age Range: | 14 weeks; drug administration since the 5th week |
Weight Or Weight Range: | 37-58 g for db/db mice; 18-25 g for wild type mice |
Gender: | Male |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000808 | AN000809 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Waters Acquity | Waters Acquity |
Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Orbitrap | Thermo Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | m/z | m/z |
Chromatography:
Chromatography ID: | CH001295 |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
Column Pressure: | 6000-10000 |
Column Temperature: | 50C |
Flow Rate: | 0.25 mL/min |
Injection Temperature: | 4C |
Internal Standard: | 1,2-diheptadecanoyl-sn-glycero-3-phosphocholine |
Solvent A: | 60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate |
Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
Analytical Time: | 30 min |
Weak Wash Solvent Name: | 60:40 water/acetonitrile in 10 mM ammonium formate with 0.1% formic acid |
Weak Wash Volume: | 1200 uL |
Strong Wash Solvent Name: | 90:10 isopropanol/acetonitrile in 10 mM ammonium formate with 0.1% formic acid |
Strong Wash Volume: | 600 uL |
Sample Loop Size: | 10 uL |
Sample Syringe Size: | 100 uL |
Randomization Order: | Yes |
Chromatography Type: | Reversed phase |