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MB Sample ID: SA028103
| Local Sample ID: | R3C-180 |
| Subject ID: | SU000565 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000825 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Zorbax Extended C18 |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE |
| Units | mole percent enrichment |
Chromatography:
| Chromatography ID: | CH000589 |
| Chromatography Summary: | UPLC separations were performed with a Dionex UltiMate 3000 system (ThermoFisher Scientific) and a Zorbax Extended C18 column (Agilent; 5 cm × 2.1 mm, 1.8 µm). Solvent A was 99% water (Fisher Scientific), 1% acetonitrile (Fisher Scientific) with 0.1% formic acid (SigmaAldrich), and solvent B was 99% acetonitrile, 1% water and 0.1% formic acid. The gradient was as follows: 0-6 min: 5-20% B; 6-10 min: 20-95% B; 10-12 min: 95% B; 12-13 min: 95-5% B, at a flow rate of 0.3 mL min−1. An injection volume of 5 µL was used. The UPLC was connected to the ion source through the diverter valve. The HESI ion source was operated with +3.5 kV spray voltage and probe heater temperature of 300 °C. Sheath, auxiliary and spare gas were 47.5, 11.25 and 1.0 (arb. units), respectively. The capillary temperature was maintained at 275 °C. |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Zorbax Extended C18 |
| Flow Gradient: | 0-6 min: 5-20% B; 6-10 min: 20-95% B; 10-12 min: 95% B; 12-13 min: 95-5% B |
| Flow Rate: | 0.3ml/min |
| Solvent A: | 99% water/1% acetonitrile; 0.1% formic acid |
| Solvent B: | 99% acetonitrile/1% water; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
