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MB Sample ID: SA030493
| Local Sample ID: | 2016008 |
| Subject ID: | SU000601 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Subject Comments: | 220 subjects |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000888 | AN000889 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Ion exchange |
| Chromatography system | Thermo Accela CTC | Thermo Accela CTC |
| Column | Thermo Higgins C18 (100 x 2.1mm,2.6um) | Hamilton Anion Exchange HPLCs,100x2.1,2.6m,Thermo Fisher |
| MS Type | ESI | ESI |
| MS instrument type | Single quadrupole | Single quadrupole |
| MS instrument name | Thermo LTQ-FT | Thermo LTQ-FT |
| Ion Mode | POSITIVE | POSITIVE |
| Units | unspecified | unspecified |
Chromatography:
| Chromatography ID: | CH000631 |
| Chromatography Summary: | Positive untargeted chromatography (C18 column) |
| Methods Filename: | Metab_Orbitrap_Setup_v2_26Jun2013.docx |
| Instrument Name: | Thermo Accela CTC |
| Column Name: | Thermo Higgins C18 (100 x 2.1mm,2.6um) |
| Column Temperature: | 30 |
| Flow Gradient: | A: 0-2mins = 90%, 2-7mins = gradient to 0%, 7-10mins = 0%. B: 0-2mins = 5%, 2-7mins = gradient to 95%, 7-10mins = 95%. C: 0-10mins = 5% |
| Flow Rate: | 350uL/min |
| Internal Standard: | Caffeine, Diethyl-toluamide |
| Internal Standard Mt: | 1.5 min, 6-6.6 min |
| Sample Injection: | 10uL |
| Sampling Cone: | HESI probe with S-lens combination for ESI |
| Solvent A: | 100% water |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 10 minutes |
| Capillary Voltage: | 4.6 kV |
| Oven Temperature: | 45 C |
| Running Voltage: | 45 |
| Weak Wash Solvent Name: | H20 with 10% methanol |
| Sample Syringe Size: | 20uL |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000632 |
| Chromatography Summary: | Positive untargeted anion exchange chromatography (AE column) |
| Methods Filename: | Metab_Orbitrap_Setup_v1_May_17_2013.docx |
| Instrument Name: | Thermo Accela CTC |
| Column Name: | Hamilton Anion Exchange HPLCs,100x2.1,2.6m,Thermo Fisher |
| Column Temperature: | 30 |
| Flow Gradient: | A: 0-2mins = 10%, 2-7mins = gradient to 80%, 7-10mins = 80%. B: 0-2mins = 80%, 2-7mins = gradient to 10%, 7-10mins = 10%. C: 0-10mins = 10% |
| Flow Rate: | 350uL/min |
| Internal Standard: | [13C6]-D-glucose, [15N]-indole, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [15N]-choline chloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,4-13C2]-cholesterol, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, [U-13C5, U-15N2]-L-glutamine |
| Sample Injection: | 10uL |
| Sampling Cone: | HESI probe with S-lens combination for ESI |
| Solvent A: | 100% water |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 10 minutes |
| Capillary Voltage: | 4.6 kV |
| Oven Temperature: | 45 C |
| Running Voltage: | 45 |
| Weak Wash Solvent Name: | H20 with 10% methanol |
| Sample Syringe Size: | 20uL |
| Chromatography Type: | Ion exchange |
