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MB Sample ID: SA035141
| Local Sample ID: | MBAL_MET143 |
| Subject ID: | SU000648 |
| Subject Type: | Human | Animal |
| Subject Species: | Homo sapiens;Mus musculus |
| Taxonomy ID: | 9606;10090 |
| Genotype Strain: | Animal: C57BL/6 |
| Species Group: | Mammal |
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Combined analysis:
| Analysis ID | AN000957 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1200 |
| Column | Agilent Zorbax Narrow Bore RRHT RRHD SB-AQ (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6520 QTOF |
| Ion Mode | POSITIVE |
| Units | peak intensity |
Chromatography:
| Chromatography ID: | CH000682 |
| Chromatography Summary: | Analysis of the aqueous fraction was by reversed-phase chromatography using an Agilent 1200 series pump with an Agilent Zorbax Narrow Bore RRHT SB-AQ (1.8 micron, 2.1 x 100 mm, 80Å) analytical column and an Agilent Zorbax SB-AQ (5 micron, 2.1 x 12.5 mm) guard column. Sample injection volumes were 10 µL. A flow rate of 0.3ml/min was used with the following mobile phases: mobile phase A was water with 0.1% formic acid, and mobile phase B was 90:10 acetonitrile:water with 0.1% formic acid. Gradient elution was as follows: 0-3 minutes 2% B, 3-5 minutes 2-40% B, 5-20 minutes 40-100% B, 20-30 minutes 100% B, 30-30.01 100-2% B, 30.01-40 minutes 2% B. |
| Instrument Name: | Agilent 1200 |
| Column Name: | Agilent Zorbax Narrow Bore RRHT RRHD SB-AQ (100 x 2.1mm,1.8um) |
| Flow Gradient: | 0-3 minutes 2% B, 3-5 minutes 2-40% B, 5-20 minutes 40-100% B, 20-30 minutes 100% B, 30-30.01 100-2% B, 30.01-40 minutes 2% B |
| Flow Rate: | 0.3ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 90% acetonitrile/10% water; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
