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MB Sample ID: SA052779
| Local Sample ID: | Control_1.3 |
| Subject ID: | SU000935 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | ATCC |
| Cell Strain Details: | Daoy (ATCC® HTB-186™) |
| Subject Comments: | cancer cell line |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001460 | AN001461 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Waters Acquity I-Class | Waters Acquity I-Class |
| Column | Waters Acquity BEH Amide (50 x 2.1mm,1.7um) | Waters Acquity BEH Amide (50 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Waters Synapt G2 QTOF | Waters Synapt G2 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Area | Peak Area |
Chromatography:
| Chromatography ID: | CH001027 |
| Chromatography Summary: | The samples were analyzed on a Acquity UPLC I-class system from Waters. A HILIC-Amide column (1.7 μm, i.d. 2.1x50 mm) from Waters was used for sample separation and a non-linear gradient elution profile from 100 % A to 100 % B was used. Mobile phase A consisted of 90:10 acetonitrile/water with 10 mM ammonium formate and 0.1% FA while mobile phase B consisted of 50:50 acetonitrile/ water with 10 mM ammonium formate and 0.1% FA. The flow rate was 0.3 ml/min, the column temperature 40°C and the injection volume 5 µL. |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters Acquity BEH Amide (50 x 2.1mm,1.7um) |
| Column Temperature: | 40°C |
| Flow Gradient: | Non-linear gradient elution profile from 100 % A to 100 % B was used. In detail; 100% A was kept for 0.5 min then decreased non-linearly (slope-factor 8 in MassLynx) over 12.5 min to 100% B, 100% B was held for 3 min followed by 7 min at 100 % A to re-equilibrate the column for a total run-time of 23 min. |
| Flow Rate: | 0.3 ml/min |
| Injection Temperature: | 4°C |
| Sample Injection: | 5 μl |
| Solvent A: | 90% acetonitrile/10% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 50% acetonitrile/50% water; 0.1% formic acid; 10 mM ammonium formate |
| Analytical Time: | 23 min total sample run-time |
| Randomization Order: | All samples were analyzed in a randomized order with three QC injections interspaced every eleventh injection |
| Chromatography Type: | HILIC |
