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MB Sample ID: SA057743
Local Sample ID: | 17 |
Subject ID: | SU001005 |
Subject Type: | Fish |
Subject Species: | Megalobrama amblycephala |
Taxonomy ID: | 75352 |
Gender: | Not applicable |
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Combined analysis:
Analysis ID | AN001580 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Unspecified |
Column | Unspecified |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | |
Ion Mode | POSITIVE |
Units | ng/ml or umol/L |
Chromatography:
Chromatography ID: | CH001109 |
Chromatography Summary: | The HPLC system consisted of a SRD-3600 Solvent Rack with analytical 6-channel vacuum degasser, a DGP-3600A pump, WPS-3000TSL analytical autosampler, and a tcc-3200 column compartment. Chromatographic separations were performed on an MSLab C18 column (150×4.6mm, 5μm). The mobile phase A was 0.1% formic acid in water, and the organic mobile phase B was 0.1% ammonium formate in acetonitrile with pH=5.8. The solvent was delivered to the column at a flow rate of 1 ml min−1 as follows: 0-1 minutes from A-B (90:10) to A-B (90:10); 1-12 minutes from A-B (90:10) to A-B (30:70); 12-12.1 minutes from A-B (30:70) to A-B (0:100); 12.1-15 minutes from A-B (0:100) to A-B (0:100); 15-15.1 minutes from A-B (0:100) to A-B (90:10); 15.1-20 minutes from A-B(90:10) to A-B (90:10). |
Instrument Name: | Unspecified |
Column Name: | Unspecified |
Flow Gradient: | 0-1 minutes from A-B (90:10) to A-B (90:10); 1-12 minutes from A-B (90:10) to A-B (30:70); 12-12.1 minutes from A-B (30:70) to A-B (0:100); 12.1-15 minutes from A-B (0:100) to A-B (0:100); 15-15.1 minutes from A-B (0:100) to A-B (90:10); 15.1-20 minutes from A-B(90:10) to A-B (90:10). |
Flow Rate: | 1ml/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% ammonium formate, pH 5.8 |
Chromatography Type: | Reversed phase |