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MB Sample ID: SA058630
Local Sample ID: | U-1043 |
Subject ID: | SU001012 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Human Ethnicity: | Yoruba |
Human Inclusion Criteria: | apparent health, no recent treatment for any disease, no recent use of antibiotics, antimalarial or other drugs |
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Combined analysis:
Analysis ID | AN001594 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Q-Exactive hybrid quadrupole orbitrap mass spectrometer |
Column | Hypersil Gold |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE |
Units | abundance of specific m/z (amu) |
Chromatography:
Chromatography ID: | CH001119 |
Chromatography Summary: | Both urine and plasma samples were prepared using chilled methanol:water mixture (4:1). Separation was achieved with 150mm x 2.1mm, 1.9μ Hypersil Gold column with a 5μl injection volume. Gradient elution was performed using 0.1% formic acid in water (A) and acetonitrile (B) as mobile phase after optimization. The column was washed between each sample for stability and to eliminate carry-over. The flow rate was 0.35 ml/min. MS acquisition was performed on the Q-Exactive hybrid quadrupole orbitrap mass spectrometer (ThermoFisher, MA, USA) operated in positive and negative electrospray ionization (ESI) modes. The scan range was 70 to 1000 m/z. |
Instrument Name: | Q-Exactive hybrid quadrupole orbitrap mass spectrometer |
Column Name: | Hypersil Gold |
Flow Rate: | 0.35ml/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |