Return to study ST000973 main page
MB Sample ID: SA058656
| Local Sample ID: | S-393 |
| Subject ID: | SU001012 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Human Ethnicity: | Yoruba |
| Human Inclusion Criteria: | apparent health, no recent treatment for any disease, no recent use of antibiotics, antimalarial or other drugs |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001594 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Q-Exactive hybrid quadrupole orbitrap mass spectrometer |
| Column | Hypersil Gold |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | abundance of specific m/z (amu) |
Chromatography:
| Chromatography ID: | CH001119 |
| Chromatography Summary: | Both urine and plasma samples were prepared using chilled methanol:water mixture (4:1). Separation was achieved with 150mm x 2.1mm, 1.9μ Hypersil Gold column with a 5μl injection volume. Gradient elution was performed using 0.1% formic acid in water (A) and acetonitrile (B) as mobile phase after optimization. The column was washed between each sample for stability and to eliminate carry-over. The flow rate was 0.35 ml/min. MS acquisition was performed on the Q-Exactive hybrid quadrupole orbitrap mass spectrometer (ThermoFisher, MA, USA) operated in positive and negative electrospray ionization (ESI) modes. The scan range was 70 to 1000 m/z. |
| Instrument Name: | Q-Exactive hybrid quadrupole orbitrap mass spectrometer |
| Column Name: | Hypersil Gold |
| Flow Rate: | 0.35ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
