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MB Sample ID: SA064536
| Local Sample ID: | D11cellE3T2 |
| Subject ID: | SU001067 |
| Subject Type: | Other |
| Subject Species: | Xenopus laevis |
| Taxonomy ID: | 8355 |
| Age Or Age Range: | Embryos were obtained from natural mating of frogs (Nasco) |
| Weight Or Weight Range: | Sexually mature male and female frogs |
| Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001686 |
|---|---|
| Analysis type | MS |
| Chromatography type | CE |
| Chromatography system | Custom built CE system |
| Column | Bare fused silica capillary |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Bruker Impact HD |
| Ion Mode | POSITIVE |
| Units | PeakAreaInCounts |
Chromatography:
| Chromatography ID: | CH001186 |
| Chromatography Summary: | About 10 nL of metabolite extract were injected into bare fused silica capillary filled with the background electrolyte, then potential difference was applied between the capillary ends to electrophoretically separate metabolites. |
| Instrument Name: | Custom built CE system |
| Column Name: | Bare fused silica capillary |
| Column Temperature: | Room temperature (~21 degC) |
| Flow Rate: | Electroosmoti flow rates apply in this study (~1 nL/min, estimated) |
| Injection Temperature: | Room temperature (~21 degC) |
| Retention Time: | Metabolites were separated across ~45 min. |
| Sample Injection: | 10 nL |
| Solvent A: | 100% water; 1% formic acid |
| Capillary Voltage: | Ca. +20,000 V applied to inlet end of the CE fused silica separation capillary |
| Migration Time: | Ca. 45 min total run time collected |
| Preconditioning: | Sodium hydroxide solution |
| Running Buffer: | See background electrolyte (above) |
| Sheath Liquid: | 50% methanol, 0.1% formic acid |
| Chromatography Type: | CE |
