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MB Sample ID: SA076770

Local Sample ID:11-Late Exponential Phase
Subject ID:SU001169
Subject Type:Bacteria
Subject Species:Acidithiobacillus ferrooxidans ATCC 23270
Taxonomy ID:243159
Genotype Strain:ATCC 23270
Cell Biosource Or Supplier:American Type Culture Collection
Cell Strain Details:ATCC 23270

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Combined analysis:

Analysis ID AN001821
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Dionex Ultimate 3000
Column SeQuant ZIC-pHILIC (150 x 2.1mm,5um)
MS Type ESI
MS instrument type QTOF
MS instrument name Bruker MicrOTOF II
Ion Mode POSITIVE
Units m/z-retention time features

Chromatography:

Chromatography ID:CH001290
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:SeQuant ZIC-pHILIC (150 x 2.1mm,5um)
Flow Gradient:(t= 0-1 minute) 3% A, 97% B; (t= 24 minute) 40% A, 60% B; (t= 28 minute) 40% A, 60% B (t= 33 minutes) 3% A, 97% B; (t= 34minutes) 3% A, 97% B. HPLC flow rate 0.4 mL/min.
Flow Rate:0.4ml/min
Solvent A:100% water; 20 mM ammonium formate
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:HILIC
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