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MB Sample ID: SA079869

Local Sample ID:WT-RD-D-011614-RT-1
Subject ID:SU001215
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:rd10 and C57BL/6J
Age Or Age Range:P18
Animal Animal Supplier:Jackson Laboratory

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Combined analysis:

Analysis ID AN001897 AN001898
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Synapt G2 Si QTOF Waters Synapt G2 Si QTOF
Ion Mode NEGATIVE POSITIVE
Units m/z_retention time m/z_retention time

Chromatography:

Chromatography ID:CH001374
Chromatography Summary:Reversed-Phase Gradient Seperation
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
Column Pressure:6,000-10,000 psi
Column Temperature:50 °C
Flow Gradient:Time(min) Flow Rate(mL/min) %A %B Curve 1. Initial 0.400 99.0 1.0 6 2. 1.00 0.400 99.0 1.0 6 3. 16.00 0.400 1.0 99.0 6 4. 20.00 0.400 1.0 99.0 6 5. 20.50 0.400 99.0 1.0 6 6. 22.00 0.400 99.0 1.0 6
Flow Rate:0.4 mL/min
Injection Temperature:8 °C
Internal Standard:L-Tryptophan-d5
Chromatography Type:Reversed phase
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