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MB Sample ID: SA081599
| Local Sample ID: | 20180922_sample_01 |
| Subject ID: | SU001243 |
| Subject Type: | Other |
| Subject Species: | Cricetulus griseus |
| Taxonomy ID: | 10029 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001953 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | ACQUITY BEH C8 column (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH001416 |
| Chromatography Summary: | Lipid extracts were separated on a Waters ACQUITY BEH C8 column (2.1 × 100 mm, 1.7 μm) with the temperature maintained at 40 °C. The flow rate was 250 μL/min, and the mobile phases were consisted of 60:40 water/acetonitrile (A), and 90:10 isopropanol/acetonitrile (B), both containing 10 mM ammonium formate and 0.1% formic acid. The samples were eluted with a linear gradient from 5 % B to 97 % B over 20 min, maintained at 97 % B for 4 min and re-equilibration with 5 % B for 6 min. The sample injection volume was 5 μL |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACQUITY BEH C8 column (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | a linear gradient from 5 % B to 97 % B over 20 min, maintained at 97 % B for 4 min and re-equilibration with 5 % B for 6 min. |
| Flow Rate: | 250 µL/min |
| Solvent A: | 60% water/40% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
