Return to study ST001181 main page
MB Sample ID: SA082010
| Local Sample ID: | 22.11_subj |
| Subject ID: | SU001247 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 16-44 years old |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001959 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH001420 |
| Chromatography Summary: | Mass spectral data were collected with a 10-minute gradient on a Thermo QExactive coupled with Dionex Ultimate 3000 (Thermo Fisher, San Diego, CA) set to collect data from m/z 85 to 1275 in positive ionization mode at 70,000 resolution. Three technical replicates were run for each sample using dual chromatography measuring both polarities (positive and negative) with a C18 column (Higgins Analytical, 100x2.1mm, 5uL). The right pump controls the gradient with Solvent A as 100% water, Solvent B as 100% Acetontrile, and Solvent C as 98% water with 2% Formic Acid. The left pump is set up to wash (Acetonitrile with 2% Formic Acid) one of the columns while the other column is running the gradient. |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| Solvent A: | 100% water(A), 100% acetontrile(B), 100% water; 2% formic acid© |
| Solvent B: | 100% water(A), 100% acetontrile(B), 100% water; 2% formic acid(C) |
| Chromatography Type: | Reversed phase |
