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MB Sample ID: SA082247
| Local Sample ID: | R1 |
| Subject ID: | SU001251 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001967 | AN001968 | AN001969 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
| Column | Thermo Fisher Accucore C18 (100 x 2.1mm,5um) | Thermo Fisher Accucore C18 (100 x 2.1mm,5um) | Phenomenex Kinetex C18+ (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | UNSPECIFIED |
| Units | Peak area normalized to internal standard | Peak area normalized to internal standard | Peak Area |
Chromatography:
| Chromatography ID: | CH001423 |
| Chromatography Summary: | LIPIDOMICS: Samples were separated on a Thermo Fisher Accucore C18 column (2.1 X 100 mm, 5 μ pore size) using solvent A (H2O:ACN (1:1) with 10 mM ammonium acetate + 0.1% formic acid) and solvent B (IPA:ACN (9:1) with 10 mM ammonium acetate + 0.1% formic acid) at a flow rate of 0.2 mL/min. The gradient started at 0%B and increased to 50%B from 2-10 min following a second increase to 95%B from 10-47 min. The gradient was held for 4 min at 95%B before increasing to 100%B at 51 min for a 6 min wash. At 57 min the system was returned to initial conditions to equilibrate before the next injection. Total run time was 60 min. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Fisher Accucore C18 (100 x 2.1mm,5um) |
| Flow Gradient: | The gradient started at 0%B and increased to 50%B from 2-10 min following a second increase to 95%B from 10-47 min. The gradient was held for 4 min at 95%B before increasing to 100%B at 51 min for a 6 min wash. At 57 min the system was returned to initial conditions to equilibrate before the next injection. Total run time was 60 min. |
| Flow Rate: | 0.2 mL/min |
| Solvent A: | 50% water/50% acetonitrile; 0.1% formic acid; 10 mM ammonium acetate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001424 |
| Chromatography Summary: | METABOLOMICS: Briefly, samples were separated over a reversed phase Phenomenex Kinetex C18+ column (2.1 × 100 mm, 1.7 μm particle size) maintained at 40°C. For the 20 minute LC gradient, the mobile phase consisted of the following: solvent A (1.5mM ammonium fluoride) and solvent B (100% acetonitrile). The gradient was the following: 0-12.0 min 5% B, to 100% B, 12.0-15.0 min hold at 100% B, 15.0-15.1 100% to 5% B, 15.1-20.0 min 5%B. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Phenomenex Kinetex C18+ (100 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
