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MB Sample ID: SA083088
Local Sample ID: | HY-3 |
Subject ID: | SU001261 |
Subject Type: | Plant |
Subject Species: | Ginkgo Biloba |
Taxonomy ID: | 3311 |
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Combined analysis:
Analysis ID | AN001990 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Shimadzu LC-30AD Series UPLC system |
Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex 5600+ TripleTOF |
Ion Mode | NEGATIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH001438 |
Chromatography Summary: | Chromatographic separation was completed on a Shimadzu LC-30AD Series UHPLC system (Shimadzu, Duisburg, Germany) equipped with supplementary SIL-30AC injector and (PAD) SPD-M20A detector. ACQUITY UPLCTM BEH C18 column (100 mm × 2.1 mm, 1.7 μm, Waters, Milford, USA) was attached to the whole analyses at 35℃. A flow rate of 0.3 mL/min was chosen to use while 0.1% formic acid water (A) and acetonitrile (B) comprising the mobile phase. The gradient elution conditions were optimized as follows:0-3 min, 5% B → 5% B; 3-25 min, 5% B → 95% B; 25-28 min, 95% B → 95% B; 28-28.1 min, 95% → 5% B, followed by 4 min re-equilibration. |
Instrument Name: | Shimadzu LC-30AD Series UPLC system |
Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
Column Temperature: | 35 |
Flow Gradient: | 0-3 min, 5% B - 5% B; 3-25 min, 5% B - 95% B; 25-28 min, 95% B - 95% B; 28-28.1 min, 95% - 5% B, followed by 4 min re-equilibration. |
Flow Rate: | 0.3ml/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |